Rapid colorimetric detection of mercury using silver nanoparticles in the presence of methionine

S Balasurya; Asad Syed; Ajith M Thomas; Najat Marraiki; Abdallah M Elgorban; Lija L Raju; Arunava Das; S Sudheer Khan

doi:10.1016/j.saa.2019.117712

Rapid colorimetric detection of mercury using silver nanoparticles in the presence of methionine

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Mar 5:228:117712. doi: 10.1016/j.saa.2019.117712. Epub 2019 Oct 30.

Authors

S Balasurya¹, Asad Syed², Ajith M Thomas³, Najat Marraiki², Abdallah M Elgorban⁴, Lija L Raju⁵, Arunava Das¹, S Sudheer Khan⁶

Affiliations

¹ Nanobiotechnology Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India.
² Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia.
³ Department of Botany and Biotechnology, St Xavier's College, Thumba, Thiruvananthapuram, India.
⁴ Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Centre of Excellence in Biotechnology Research, King Saud University, P.O Box 2455, Riyadh, 11451, Saudi Arabia.
⁵ Department of Zoology, Mar Ivanios College, Nalanchira, Thiruvananthapuram, India.
⁶ Nanobiotechnology Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India. Electronic address: sudheerkhan@bitsathy.ac.in.

PMID: 31753653
DOI: 10.1016/j.saa.2019.117712

Abstract

Development of potential sensors is inevitable for the detection of environmental pollutants including toxins, organic pollutants and heavy metal which cause hazardous effect to human and other living organisms. The present study is to develop silver nanoparticle (Ag NPs) based sensor for the accurate, sensitive and selective colorimetric detection of Hg²⁺ ions from aqueous samples at nano molar level. The nanoparticles were synthesized chemically and it was stabilized by polyvinylpyrrolidone (PVP). The prepared particles were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), particle analysiser and Zetasizer. The UV-visible spectra of Ag NPs showed absorbance maximum at 392 nm. The average diameter of Ag NPs was determined to be 6 ± 0.9 nm by using particle analyzer. The zeta sizer analysis showed that the PVP stabilized Ag NPs possessed a zeta potential of -35.56 ± 3 mV. The Ag NPs-methionine conjugate showed the colour change from the brownish yellow colour to colourless when it was reacted with mercury. The Ag NPs conjugated methionine is sensitive to mercury and detects the mercury at nano molar level. The influence of other metal ion did not interfere with the detection and quantification of Hg²⁺. The detection of Hg²⁺ was also performed with paper strip and agarose gel method. The Ag NPs conjugate with methionine can applied for the detection of Hg²⁺ from various aqueous samples.

Keywords: Ag NPs; Ag NPs-methionine conjugate; Detection of Hg(2+); PVP.