Targeting delivery of partial VAR2CSA peptide guided N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles for multiple cancer types

Kai Zhao; Guogang Cheng; Baozhen Zhang; Dan Li; Jinyu Han; Jie Chen; Baobei Wang; Mengxia Li; Tianxia Xiao; Aiwen Le; Beini Sun; Zheng Jin; Jian Zhang; Xiujun Fan

doi:10.1016/j.msec.2019.110171

Targeting delivery of partial VAR2CSA peptide guided N-2-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles for multiple cancer types

Mater Sci Eng C Mater Biol Appl. 2020 Jan:106:110171. doi: 10.1016/j.msec.2019.110171. Epub 2019 Sep 6.

Authors

Kai Zhao¹, Guogang Cheng², Baozhen Zhang³, Dan Li⁴, Jinyu Han⁵, Jie Chen⁶, Baobei Wang⁶, Mengxia Li⁶, Tianxia Xiao⁶, Aiwen Le⁷, Beini Sun⁸, Zheng Jin⁹, Jian Zhang⁶, Xiujun Fan¹⁰

Affiliations

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin 150080, China. Electronic address: zhaokai@hlju.edu.cn.
² Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin 150080, China; Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China.
³ Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China; University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin 150080, China; Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China.
⁵ Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China; Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, Harbin 150080, China.
⁶ Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China.
⁷ Department of Obstetrics and Gynaecology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen 518052, China.
⁸ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin 150080, China.
⁹ Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, Harbin 150080, China.
¹⁰ Center for Reproduction and Health Development, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518052, China. Electronic address: xj.fan@siat.ac.cn.

PMID: 31753378
DOI: 10.1016/j.msec.2019.110171

Abstract

To developing a multiple cancer types targeting drug delivery carrier system, a 28 amino acids from the VAR2CSA was synthesized as the placental CSA-binding peptide (plCSA-BP). Its specific binding ability to cancer cells was tested on cancer tissue array, and the results showed that plCSA-BP could bind to multiple cancer types. Then, the plCSA-BP was used as a guiding peptide to coat nanoparticles synthesized from N-2-HACC (CSA/HACC-NPs) which were loaded with prodigiosin (CSA/HACC-PNPs) or indocyanine green (CSA/HACC-INPs). The cancer cells specific targeting and efficacy of the CSA/HACC-PNPs were tested by different cancer cells in vitro and various cancer xenograft model in vivo. A scramble peptide (SCR) was used as control and synthesized SCR/HACC-PNPs and SCR/HACC-INPs. The results showed that the CSA/HACC-INPs could specifically uptake by JEG-3, PC3 and A594 cells, and the CSA/HACC-PNPs exhibited better anti-cancer activity and lower toxic effect in subcutaneous choriocarcinoma and prostatic tumor models compared with the free prodigiosin, HACC-PNPs and SCR/HACC-PNPs. So, the CSA/HACC-NPs could be used as a specific delivery carrier for multiple cancer types, and provided an alternate treatment option of various cancers with a single recipe.

Keywords: Drug delivery; Multiple cancer types targeting; N-2-Hydroxypropyl trimethyl ammonium chloride chitosan; Nanoparticles; plCSA-BP.

MeSH terms

Cell Line, Tumor
Chitosan / analogs & derivatives*
Chitosan / chemistry*
Drug Carriers / chemistry
Drug Delivery Systems / methods
Female
Humans
Male
Nanoparticles / chemistry*
PC-3 Cells

Substances

Drug Carriers
chitosan-N-2-hydroxypropyl trimethyl ammonium chloride
Chitosan