Male rainbow darters (Etheostoma caeruleum) prefer larger conspecific females

Abstract

Female mate choice is a well studied mechanism of sexual selection that can affect the evolution of male secondary sexual traits. However, males also exhibit selective mate choice in some animals, which can affect the evolution of female phenotypes. We tested for evidence of intraspecific male mate choice in the Rainbow Darter (Percidae: Etheostoma caeruleum). Males of numerous darter species have been shown to prefer conspecific over heterospecific females; however, intraspecific preferences in males have not yet been demonstrated in this genus. This study investigated male preference for indicators of female fecundity, specifically female size. We found that male Rainbow Darters prefer larger females, both in dichotomous choice tests, when access between individuals was prevented, and in unrestricted behavioral tests with full access to females. Notably, however, the dichotomous trials were not repeatable; males only displayed a significant preference for larger females in the first dichotomous trial and in the unrestricted trial. We discuss our results in light of experimental methods and the role of male mate choice in sexual selection.