Abstract
Here, we designed and developed a Universal Baby Spinach-based Probe (UBSP) for biomolecule detection by introducing a DNA repressor containing a target recognition element. By employing different interaction modes between targets and repressors, we applied the UBSP to detect diverse classes of analytes, including microRNA, proteins, and heavy metal ions.
MeSH terms
-
Aptamers, Nucleotide / chemistry*
-
Benzyl Compounds / chemistry*
-
Biosensing Techniques / methods
-
Blood Proteins / analysis
-
DNA / chemistry
-
DNA Probes / chemistry*
-
Fluorescent Dyes / chemistry*
-
G-Quadruplexes
-
Humans
-
Imidazolines / chemistry*
-
Mercury / analysis
-
MicroRNAs / analysis
-
RNA / chemistry
-
RNA Probes / chemistry*
-
RNA Probes / genetics
-
Spectrometry, Fluorescence / methods
Substances
-
3,5-difluoro-4-hydroxybenzylidene imidazolinone
-
Aptamers, Nucleotide
-
Benzyl Compounds
-
Blood Proteins
-
DNA Probes
-
Fluorescent Dyes
-
Imidazolines
-
MIRN21 microRNA, human
-
MicroRNAs
-
RNA Probes
-
RNA
-
DNA
-
Mercury