MicroRNA-96-3p promotes metastasis of papillary thyroid cancer through targeting SDHB

Xupeng Zhao; Yingjie Li; Yong Zhou

doi:10.1186/s12935-019-1003-y

MicroRNA-96-3p promotes metastasis of papillary thyroid cancer through targeting SDHB

Cancer Cell Int. 2019 Nov 12:19:287. doi: 10.1186/s12935-019-1003-y. eCollection 2019.

Authors

Xupeng Zhao¹, Yingjie Li², Yong Zhou¹

Affiliations

¹ 1Department of Fourth General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032 China.
² 2Department of Sixth General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032 China.

Abstract

Background: MicroRNA (MiRNA) is a small non-coding RNA which is implicated in a cohort of biological function in cancer, including proliferation, metastasis, apoptosis and invasion. MiR-96 has been reported to be involved in many cancers, including papillary thyroid cancer. However, the role of miR-96-3p in papillary thyroid cancer metastasis is still unclear.

Methods: qRT-PCR is used to detect the level of miR-96-3p and mRNA of SDHB in PTC tissues and cell lines. Western blot assays are used to verify the protein expression of SDHB. The transwell assays are performed to identify the migration ability of PTC cell lines. Moreover, dual-luciferase 3'-UTR reporter assays are chosen to illuminate the direct target of miR-96-3p.

Results: The relative miR-96-3p upregulate in PTC tissues and three PTC cell lines (B-CPAP, K-1 and TPC-1 cells) while the relative SDHB is opposite. Our results revealed that the miR-96-3p promotes metastasis and invasion in PTC cell lines (K-1 and TPC-1 cells) by direct targeting SDHB and influence the downstream protein AKT.

Conclusions: Taken together, the miR-96-3p is involved in PTC metastasis and invasion by direct targeting SDHB and the downstream molecule AKT and mTOR.

Keywords: AKT pathway; MicroRNA-96-3p; Papillary thyroid cancer; SDHB.