Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn., in vitro, and on neutrophil nitro-oxidative functions

Nabila Tounsi; Bahia Djerdjouri; Ouahiba Ait Yahia; Aicha Belkebir

doi:10.1007/s13197-019-04021-z

Pro-oxidant versus anti-oxidant effects of seeds aglycone extracts of Lepidium sativum and Eruca vesicaria Linn., in vitro, and on neutrophil nitro-oxidative functions

J Food Sci Technol. 2019 Dec;56(12):5492-5499. doi: 10.1007/s13197-019-04021-z. Epub 2019 Aug 14.

Authors

Nabila Tounsi¹, Bahia Djerdjouri¹, Ouahiba Ait Yahia², Aicha Belkebir²

Affiliations

¹ 1Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Sciences and Technology, Houari Boumediene, BP 32, El-Alia, Bab-Ezzouar, 16111 Algiers, Algeria.
² 2Laboratory of Plant Physiology, Faculty of Biological Sciences, University of Sciences and Technology, Houari Boumediene, Algiers, Algeria.

Abstract

This study evaluated the anti-inflammatory and antioxidant properties of seeds aglycone extracts from Lepidium sativum (LS) and Eruca vesicaria (EV) Linn., on oxidative damages in vitro and on neutrophil nitro-oxidative functions. The results showed that LS and EV aglycone extracts attenuated liver microsomal lipids and proteins oxidation through a potent antioxidant effect as attested by the dose dependent quenching of DPPH radical scavenging activity. LS and EV aglycone extracts inhibited dose dependently the production of superoxide anion by BALB/c mice-derived peritoneal neutrophils, whereas they slightly enhanced exocytosis of myeloperoxidase (MPO), a marker of azurophilic granules. Interestingly, only LS replenished glutathione (GSH) and nitric oxide levels, indicating a fine differential effect. This study highlighted the subtle oxidative and antioxidant capacity of LS and EV seeds aglycone extracts. These health promoting compounds could be used to finely modulate critical events involved in microbial infection, inflammation and nitro-oxidative stress.

Keywords: Eruca vesicaria; Glutathione; Lepidium sativum; Lipid peroxides; Myeloperoxidase; Nitro-oxidative stress.