Combining dielectrophoresis and computer vision for precise and fully automated single-cell handling and analysis

Lab Chip. 2019 Dec 21;19(24):4016-4020. doi: 10.1039/c9lc00800d. Epub 2019 Nov 20.

Abstract

With the advent of single-cell technologies comes the necessity for efficient protocols to process single cells. We combine dielectrophoresis with open source computer vision programming to automatically control the trajectories of single cells inside a microfluidic device. Using real-time image analysis, individual cells are automatically selected, isolated and spatially arranged.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis* / instrumentation
  • Electrophoresis* / methods
  • Equipment Design
  • Lab-On-A-Chip Devices*
  • Microfluidic Analytical Techniques*
  • Single-Cell Analysis* / instrumentation
  • Single-Cell Analysis* / methods