Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF

Methods Mol Biol. 2020:2080:147-158. doi: 10.1007/978-1-4939-9936-1_13.

Abstract

MIF has been described to be associated with autophagy in a number of studies, but the full nature of their association is not yet clear. However, the unprecedented interest in autophagy in recent times has generated a number of tools and techniques for its study. Here, we present protocols for studying the interactions between MIF and autophagy, including for the induction and inhibition of autophagy and measuring autophagosome biogenesis and maturation.

Keywords: Autophagosome; LC3; Macroautophagy; Rapamycin; SQSTM1/p62; siRNA.

MeSH terms

  • Animals
  • Autophagosomes / metabolism
  • Autophagy / drug effects
  • Autophagy / genetics*
  • Autophagy / immunology
  • Carrier Proteins
  • Gene Knockdown Techniques
  • Macrophage Migration-Inhibitory Factors / genetics*
  • Macrophage Migration-Inhibitory Factors / metabolism*
  • Macrophages / drug effects
  • Macrophages / immunology
  • Macrophages / metabolism
  • Mice
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phosphoinositide-3 Kinase Inhibitors / pharmacology
  • Protein Binding
  • Protein Kinase Inhibitors / pharmacology
  • RNA, Small Interfering / genetics

Substances

  • Carrier Proteins
  • Macrophage Migration-Inhibitory Factors
  • Phosphoinositide-3 Kinase Inhibitors
  • Protein Kinase Inhibitors
  • RNA, Small Interfering