[Antimicrobial resistance and clinical significant resistance mechanisms of Salmonella isolated in 2014-2018 in St.Petersburg, Russia]

S A Egorova; L A Kaftyreva; L V Suzhaeva; A V Zabrovskaia; E V Voitenkova; Z N Matveeva; Y V Ostankova; I V Likhachev; N V Satosova; R V Kitsbabashvili; E V Smirnova; L I Semchenkova; T E Bystraya; S E Sokol'nik; N P Utkina; L Y Sikhando

doi:10.18821/0869-2084-2019-64-10-620-626

[Antimicrobial resistance and clinical significant resistance mechanisms of Salmonella isolated in 2014-2018 in St.Petersburg, Russia]

Klin Lab Diagn. 2019;64(10):620-626. doi: 10.18821/0869-2084-2019-64-10-620-626.

[Article in Russian]

Authors

S A Egorova¹, L A Kaftyreva^{1

2}, L V Suzhaeva¹, A V Zabrovskaia¹, E V Voitenkova¹, Z N Matveeva¹, Y V Ostankova¹, I V Likhachev¹, N V Satosova², R V Kitsbabashvili³, E V Smirnova⁴, L I Semchenkova⁴, T E Bystraya⁴, S E Sokol'nik⁵, N P Utkina⁵, L Y Sikhando⁵

Affiliations

¹ Saint-Petersburg Pasteur Institute, 197101, Saint-Petersburg, Russia.
² State Educational Institution of the Higher Professional Education «North-Western state medical University n.a. I.I. Mechnikov» of the Ministry of Health of the Russian Federation, 191015, Saint-Petersburg, Russia.
³ ANO «Medical Center XXI Century», 194044, Saint-Petersburg, Russia.
⁴ FBUZ «Center for Hygiene and Epidemiology in St. Petersburg», division № 3, 192012, Saint-Petersburg, Russia.
⁵ FBUZ «Center for Hygiene and Epidemiology in St. Petersburg», division № 6, 198329, Saint-Petersburg, Russia.

PMID: 31742956
DOI: 10.18821/0869-2084-2019-64-10-620-626

Abstract

The article presents the results of antimicrobial resistance monitoring of Salmonella isolated from children and adults with diarrhea in St. Petersburg in 2014-2018. In 746 isolates of 42 serovars more than 90,0% belonged to three: S. enteritidis (79,6%), S. typhimurium (6,8%) and S. infantis (3,8%). The antimicrobial susceptibility testing (according the EUCAST) to 7 classes of antimicrobials revealed the resistance in 78,6% of Salmonella. Low-level quinolone resistance (MIC of ciprofloxacin 0,12-0,5 mg/l) was detected in 63,3% isolates (S. enteritidis -71,0%, S. typhimurium - 15,7%, S. infantis - 89,3%) and was due to five kinds of single nucleotide substitutions in gyrA: Asp87Tyr - 36,1% of studied isolates (only S. infantis); Ser83Phe - 22,2% (only S. enteritidis); Asp87Asn - 19,4% (S. enteritidis, S. typhimurium, S. hadar, S. newport); Ser83Tyr -11,1% (S. enteritidis and S. infantis) and Asp87Gly - 8,3% (only S. enteritidis). Only in one S. kentucky isolate with high-level fluoroquinolone resistance (MIC of ciprofloxacin > 8,0 mg/l) two substitutions (Ser83Phe and Asp87Asn) were detected. Two Salmonella isolates (S. typhimurium and S. corvallis) had plasmid-mediated quinolone resistance (qnrS). Extended-spectrum cephalosporin resistance was found in 6 Salmonella serovars (1,6%). The bla-genes were detected: of genetic group CTX-M1 - in 10 isolates (serovars S. typhimurium, S. enteritidis, S. abony, S. coeln and S. virchow), CTX-M2 - in 2 S. typhimurium isolates, CTX-M9 - in three S. enteritidis isolates. In one S. typhimurium CTX-M1 and CTX-M2 were detected. The gene of CMY-2 (molecular class C cephalosporinase) was revealed in two isolates (S. newport and S. enteritidis). Our study showed that Salmonella (the main bacterial pathogen of acute diarrhea in children and adults) isolated in Saint-Petersburg had antimicrobial resistance to drugs of choice for salmonellosis treatment.

Keywords: AmpC; ESBL; Salmonella; beta-lactamases; cephalosporins; gyrA; qnrS; quinolones; resistance.

MeSH terms

Adult
Anti-Bacterial Agents / pharmacology*
Child
Drug Resistance, Bacterial*
Humans
Microbial Sensitivity Tests
Russia
Salmonella / drug effects*

Substances

Anti-Bacterial Agents