TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast

Shamsul Morshed; Tasnuva Sharmin; Takashi Ushimaru

doi:10.1016/j.bbrc.2019.11.064

TORC1 regulates ESCRT-0 complex formation on the vacuolar membrane and microautophagy induction in yeast

Biochem Biophys Res Commun. 2020 Jan 29;522(1):88-94. doi: 10.1016/j.bbrc.2019.11.064. Epub 2019 Nov 15.

Authors

Shamsul Morshed¹, Tasnuva Sharmin¹, Takashi Ushimaru²

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
² Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address: ushimaru.takashi@shizuoka.ac.jp.

PMID: 31740006
DOI: 10.1016/j.bbrc.2019.11.064

Abstract

Microautophagy is promoted after nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) kinase. Invagination of vacuolar membranes by endosomal sorting complex required for transport (ESCRT) is required for microautophagy. Vps27, a subunit of ESCRT-0, is recruited onto vacuolar membranes via dephosphorylation after TORC1 inactivation. Here, we showed that Hse1, another ESCRT-0 subunit, is also recruited onto vacuolar membranes after TORC1 inactivation, promoting formation of ESCRT-0 complex on vacuolar membranes. Hse1 recruitment was dependent on Vps27, whereas Vps27 recruitment was independent of Hse1. Not only Vps27 but also Hse1 was required for ESCRT-III recruitment onto vacuolar membranes and microautophagy induction after TORC1 inactivation. This study revealed that ESCRT-0 (Vps27-Hse1) complex formation on vacuolar membranes is important for microautophagy inactivation after TORC1 inactivation.

Keywords: ESCRT-0; Hse1; Microautophagy; TORC1; Vps27.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Endosomal Sorting Complexes Required for Transport / metabolism*
Endosomes / metabolism
Green Fluorescent Proteins / metabolism
Intracellular Membranes / metabolism
Microautophagy*
Protein Transport
Receptors, Cytoplasmic and Nuclear / metabolism
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Transcription Factors / metabolism*
Vacuoles / metabolism*

Substances

Endosomal Sorting Complexes Required for Transport
Hse1 protein, S cerevisiae
Receptors, Cytoplasmic and Nuclear
Saccharomyces cerevisiae Proteins
TORC1 protein complex, S cerevisiae
Transcription Factors
VPS27 protein, S cerevisiae
Green Fluorescent Proteins