Silver Nanoparticles Induce Mitochondrial Protein Oxidation in Lung Cells Impacting Cell Cycle and Proliferation

Reetta J Holmila; Stephen A Vance; S Bruce King; Allen W Tsang; Ravi Singh; Cristina M Furdui

doi:10.3390/antiox8110552

Silver Nanoparticles Induce Mitochondrial Protein Oxidation in Lung Cells Impacting Cell Cycle and Proliferation

Antioxidants (Basel). 2019 Nov 14;8(11):552. doi: 10.3390/antiox8110552.

Authors

Reetta J Holmila¹, Stephen A Vance², S Bruce King², Allen W Tsang¹, Ravi Singh³, Cristina M Furdui¹

Affiliations

¹ Department of Internal Medicine, Section on Molecular Medicine, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.
² Department of Chemistry, Wake Forest University, Winston-Salem, NC 27109, USA.
³ Department of Cancer Biology, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.

Abstract

Silver nanoparticles (AgNPs) are widely used nanomaterials in both commercial and clinical biomedical applications, due to their antibacterial properties. AgNPs are also being explored for the treatment of cancer in particular in combination with ionizing radiation. In this work, we studied the effects of AgNPs and ionizing radiation on mitochondrial redox state and function in a panel of lung cell lines (A549, BEAS-2B, Calu-1 and NCI-H358). The exposure to AgNPs caused cell cycle arrest and decreased cell proliferation in A549, BEAS-2B and Calu-1, but not in NCI-H358. The mitochondrial reactive oxygen species (ROS) and protein oxidation increased in a time- and dose-dependent manner in the more sensitive cell lines with the AgNP exposure, but not in NCI-H358. While ionizing radiation also induced changes in the mitochondrial redox profiles, in general, these were not synergistic with the effects of AgNPs with the exception of NCI-H358 and only at a higher dose of radiation.

Keywords: cell cycle; mitochondria; protein oxidation; silver nanoparticles.

Abstract

Grants and funding