Scan-Rescan Repeatability and Impact of B0 and B1 Field Nonuniformity Corrections in Single-Point Whole-Brain Macromolecular Proton Fraction Mapping

Abstract

Background: Single-point macromolecular proton fraction (MPF) mapping is a recent quantitative MRI method for fast assessment of brain myelination. Information about reproducibility and sensitivity of MPF mapping to magnetic field nonuniformity is important for clinical applications.

Purpose: To assess scan-rescan repeatability and a value of B₀ and B₁ field inhomogeneity corrections in single-point synthetic-reference MPF mapping.

Study type: Prospective.

Population: Eight healthy adult volunteers underwent two scans with 11.5 ± 2.3 months interval.

Field strength/sequence: 3T; whole-brain 3D MPF mapping protocol included three spoiled gradient-echo sequences providing T₁ , proton density, and magnetization transfer contrasts with 1.25 × 1.25 × 1.25 mm³ resolution and B₀ and B₁ mapping sequences.

Assessment: MPF maps were reconstructed with B₀ and B₁ field nonuniformity correction, B₀ - and B₁ -only corrections, and without corrections. Mean MPF values were measured in automatically segmented white matter (WM) and gray matter (GM).

Statistical tests: Within-subject coefficient of variation (CV), intraclass correlation coefficient (ICC), Bland-Altman plots, and paired t-tests to assess scan-rescan repeatability. Repeated-measures analysis of variance (ANOVA) to compare field corrections.

Results: Maximal relative local MPF errors without correction in the areas of largest field nonuniformities were about 5% and 27% for B₀ and B₁ , respectively. The effect of B₀ correction was insignificant for whole-brain WM (P > 0.25) and GM (P > 0.98) MPF. The absence of B₁ correction caused a positive relative bias of 4-5% (P < 0.001) in both tissues. Scan-rescan agreement was similar for all field correction options with ICCs 0.80-0.81 for WM and 0.89-0.92 for GM. CVs were 1.6-1.7% for WM and 0.7-1.0% for GM.

Data conclusion: The single-point method enables high repeatability of MPF maps obtained with the same equipment. Correction of B₀ inhomogeneity may be disregarded to shorten the examination time. B₁ nonuniformity correction improves accuracy of MPF measurements at 3T. Reliability of whole-brain MPF measurements in WM and GM is not affected by B₀ and B₁ field corrections.

Level of evidence: 2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2020;51:1789-1798.

Keywords: B0 mapping; B1 mapping; macromolecular proton fraction; myelin; reproducibility.