QTLs position of some important ornamental traits in recently developed OO lily population

Younes Pourbeyrami Hir; SuXia Yuan; Mousa Torabi Giglou; Ming Jun

doi:10.1007/s12298-019-00688-y

QTLs position of some important ornamental traits in recently developed OO lily population

Physiol Mol Biol Plants. 2019 Nov;25(6):1419-1434. doi: 10.1007/s12298-019-00688-y. Epub 2019 Sep 7.

Authors

Younes Pourbeyrami Hir^{1

2}, SuXia Yuan², Mousa Torabi Giglou¹, Ming Jun²

Affiliations

¹ 1Horticultural Department of Agriculture and Natural Resources Faculty, Mohaghegh Ardabili University, Ardabil, Islamic Republic of Iran.
² 2Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081 People's Republic of China.

Abstract

Lilium L. is a perennial ornamental bulbous species, belonging to Liliaceae family, which consists of about 100 species. One of the most important hybrids in Lilium L. is the Oriental hybrid lily. Different cross combinations have been done in the lily family such as AA (Asiatic × Asiatic), AL (Asiatic × Longiflorum), and OT (Oriental × Trumpet). The OO (Oriental × Oriental) combination is a new one. SSR and AFLP markers were used to overlap each other and the genetic linkage groups were created according to the haploid number of lily chromosomes (12 linkage groups). In this experiment, the final F₁ population, which creates a genetic linkage group, was 100 individuals. For map construction, JOINMAP 4.0 software by treating segregation data of markers as a CP (out breeder full-sib family) model was used. After evaluation of ornamental traits, MapQTL 4.0 software was also used to find possible QTLs on these linkage maps. A total of 940 primers were tested and the best ones, which were 172 primer pairs (96 AFLP and 76 SSR markers), were used for map construction and the total of 616 loci (465 loci for AFLP marker and 151 loci for SSR marker) were scored. The entire mapped length was 2144.2 cM. 8 QTLs were obtained for flower number which is an important trait in lily. Each QTL locus explained the phenotypic variation of 2.4-89.5%. The highest amount of LOD (35.21) was found in LG-F1P2 for FN4 QTL. For leaf number, one-QTL was mapped with LOD of 7.08 between 2 markers on the LG-M10 of maternal maps. The QTL for petal length was placed on the LG-F1P2 of the F1 hybrid maps on the E-CGC/M-CGC-4 primer combination. The petal width QTLs also were mapped on the E-CGC/M-CGC-4. Qualitative locus named LN was mapped on the LG-M10 of the maternal maps. PW2 QTL was also localized on the LG-F4 of the paternal maps. In this experiment, 5 QTLs also were mapped for spot number in all F₁ hybrids and paternal and maternal maps, and spot size. Moreover, one QTL with the length of 51 cM was measured on the LG-M8 of the maternal maps. Plant height QTL with the LOD of 12.54 was mapped on the primer combination of E-CGC/M-CGC-4 on the LG-F1P2 of the F₁ hybrid maps.

Keywords: Chromosomes; Linkage map; Loci; QTL; Spot.