Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m5U formation in both tRNA and rRNA

Ivan Laptev; Ekaterina Shvetsova; Sergey Levitskii; Marina Serebryakova; Maria Rubtsova; Alexey Bogdanov; Piotr Kamenski; Petr Sergiev; Olga Dontsova

doi:10.1080/15476286.2019.1694733

Mouse Trmt2B protein is a dual specific mitochondrial metyltransferase responsible for m⁵U formation in both tRNA and rRNA

RNA Biol. 2020 Apr;17(4):441-450. doi: 10.1080/15476286.2019.1694733. Epub 2019 Nov 27.

Authors

Ivan Laptev^{1

2}, Ekaterina Shvetsova³, Sergey Levitskii⁴, Marina Serebryakova^{1

5}, Maria Rubtsova^{1

2}, Alexey Bogdanov^{2

5}, Piotr Kamenski⁴, Petr Sergiev^{1

2

5

6}, Olga Dontsova^{1

2

5

7}

Affiliations

¹ Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow Region, Russia.
² Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
³ Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.
⁴ Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
⁵ Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
⁶ Institute of Functional Genomics, Lomonosov Moscow State University, Moscow, Russia.
⁷ Department of Functioning of Living Systems, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.

Abstract

RNA molecules of all species contain modified nucleotides and particularly m⁵U residues. The vertebrate mitochondrial small subunit rRNA contains m⁵U nucleotide in a unique site. In this work we found an enzyme, TRMT2B, responsible for the formation of this nucleotide and m⁵U residues in a number of mitochondrial tRNA species. Inactivation of the Trmt2B gene leads to a reduction of the activity of respiratory chain complexes I, III and IV, containing the subunits synthesized by the mitochondrial protein synthesis apparatus. Comparative sequence analysis of m⁵U-specific RNA methyltransferases revealed an unusual evolutionary pathway of TRMT2B formation which includes consecutive substrate specificity switches from the large subunit rRNA to tRNA and then to the small subunit rRNA.

Keywords: RNA methyltransferase; mitochondria; rRNA; ribosome; tRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cell Line
Electron Transport Complex I / metabolism
Electron Transport Complex III / metabolism
Methylation
Mice
Mitochondria / enzymology*
Mitochondria / genetics
Mitochondria / metabolism
Nucleic Acid Conformation
RNA, Mitochondrial / chemistry
RNA, Mitochondrial / metabolism
RNA, Ribosomal / chemistry
RNA, Ribosomal / metabolism*
RNA, Transfer / chemistry
RNA, Transfer / metabolism*
Substrate Specificity
Thymine / metabolism
tRNA Methyltransferases / genetics
tRNA Methyltransferases / metabolism*

Substances

RNA, Mitochondrial
RNA, Ribosomal
RNA, ribosomal, 12S
RNA, Transfer
tRNA Methyltransferases
Electron Transport Complex I
Electron Transport Complex III
Thymine

Grants and funding

This work was supported by the Russian Science Foundation [19-14-00043]; Russian Foundation for Basic Research [18-29-07002, 17-00-00366].