Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle

Vanja Stojić; Branislav Glišić; Ljiljana Djukić; Bogomir Prokić; Aleksa Janović; Zorana Stamenković; Sanja Milutinović-Smiljanić; Vesna Danilović; Božidar Brković; Jelena Roganović

doi:10.1016/j.archoralbio.2019.104599

Mandibular lateral deviation induces alteration in vascular endothelial growth factor expression and oxidative stress/nitric oxide generation in rat condyle, synovial membrane and masseter muscle

Arch Oral Biol. 2020 Feb:110:104599. doi: 10.1016/j.archoralbio.2019.104599. Epub 2019 Nov 9.

Affiliations

¹ Department of Orthodontics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
² Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
³ Department for Surgery, Orthopedics and Ophtalmology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia.
⁴ Department of Diagnostic Radiology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
⁵ Department of Histology, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
⁶ Department of Oral surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.
⁷ Department of Pharmacology in Dentistry, School of Dental Medicine, University of Belgrade, Belgrade, Serbia. Electronic address: jelena.roganovic@stomf.bg.ac.rs.

PMID: 31734543
DOI: 10.1016/j.archoralbio.2019.104599

Abstract

Objective: We aimed to investigate alteration in cellular signaling mediated by vascular endothelial growth factor (VEGF) and parameters of oxidative stress/nitric oxide generation, superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS), underlying altered functional mechanical loading of TMJ (temporomandibular joint) during lateral mandibular deviation.

Design: Thirty-eight 5-week-old male Wistar rats were divided into experimental group, which received acrylic resin appliance that shifted mandible to the left during closure, and control group. Computed tomography and histomorphometry were used for condyle analyses, while samples of condyle, synovial membrane and m. masseter were analyzed with enzyme-linked immunosorbent assay and spectrophotometry to determine VEGF and nNOS protein concentrations, and SOD activity.

Results: Experimental group of rats developed smaller and asymmetrical mandibles. Less of new bone and cartilage formation and larger bone marrow cavities area were found in the experimental group. Higher VEGF expression in condyle and m. masseter as well as higher nNOS expression in m. masseter and synovial membrane were found in the experimental compared to the control group. Alteration of SOD activity was found in m. masseter and synovial membrane in the experimental group.

Conclusions: Lateral mandibular deviation induces mandibular and condylar morphological changes as well as significant cellular signaling alterations in condyle, synovial membrane and masticatory muscle. Cellular VEGF protein overexpression and oxidative stress/nitric oxide disbalance could be the mechanisms underlying unbalanced functional TMJ loading due to mandibular deviation.

Keywords: Condyle; Lateral mandibular deviation; Oxidative stress; Synovial membrane; VEGF; m. Masseter; nNOS.

MeSH terms

Animals
Male
Mandible / metabolism
Mandibular Condyle* / metabolism
Masseter Muscle* / metabolism
Nitric Oxide
Oxidative Stress*
Rats
Rats, Wistar
Synovial Membrane* / metabolism
Vascular Endothelial Growth Factor A* / metabolism

Substances

Vascular Endothelial Growth Factor A
vascular endothelial growth factor A, rat
Nitric Oxide