A bacterial strain of Pseudomonas aeruginosa B0406 pathogen opportunistic, produce a biosurfactant with tolerance to changes of pH, salinity and temperature

Gilberto Somoza-Coutiño; Arnoldo Wong-Villarreal; Cristina Blanco-González; Bianca Pérez-Sariñana; Martha Mora-Herrera; Silvia Ivonne Mora-Herrera; Raymundo Rene Rivas-Caceres; Nadia de la Portilla-López; Jorge Lugo; Rocío Vaca-Paulín; Pedro Del Águila; Gustavo Yañez-Ocampo

doi:10.1016/j.micpath.2019.103869

A bacterial strain of Pseudomonas aeruginosa B0406 pathogen opportunistic, produce a biosurfactant with tolerance to changes of pH, salinity and temperature

Microb Pathog. 2020 Feb:139:103869. doi: 10.1016/j.micpath.2019.103869. Epub 2019 Nov 14.

Affiliations

¹ Master of Renewable Energy, Polytechnic University of Chiapas, 29150, Mexico.
² Agrifood Division Technological University of the Forest, Ocosingo, Chiapas, 29950, Mexico. Electronic address: wova79@hotmail.com.
³ Academic of Applied Environmental Engineering, Polytechnic University of Chiapas, 29150, Mexico.
⁴ Academic of Applied Energy Engineering, Polytechnic University of Chiapas, 29150, Mexico.
⁵ Universitary Center Tenancingo, Autonomous University of the State of Mexico, 50000, Mexico.
⁶ Metabolomic and Proteomic Unit, Biomedical Research Institute, National Autonomous University of Mexico, 04510, Mexico.
⁷ Autonomous University of Ciudad Juárez, 32310, Mexico.
⁸ Laboratory of Edaphology and Environment, Faculty of Sciences, Autonomous University of the State of Mexico, 50000, Mexico.
⁹ Laboratory of Edaphology and Environment, Faculty of Sciences, Autonomous University of the State of Mexico, 50000, Mexico. Electronic address: gyanezo@uaemex.mx.

PMID: 31734386
DOI: 10.1016/j.micpath.2019.103869

Abstract

A bacterial strain of Pseudomonas aeruginosa B0406 catalogued as pathogen opportunistic was capable to grow with waste cooking oil as only carbon source and produce a biosurfactant. Stability to pH (from 2 to 12), salinity (% NaCl from 0 to 20%) and temperature (from -20 °C up to 120 °C), of biosurfactants was evaluated using a response surface methodology. Biosurfactants reduced surface tension from 50 to 29 ± 1.0 mN/m. Pseudomonas aeruginosa B0406 showed a high biosurfactant yield 4.17 g/L ± 0.38. Biosurfactants stability applying a response surface methodology was observed with combining effect of pH, salinity and temperature. The three factors combined do not affect surface tension of biosurfactants produced by Pseudomonas aeruginosa B0406. Therefore, this biosurfactants are of interest for medical, cosmetic even environmental applications.

Keywords: Biosurfactants; Pathogenic Pseudomonas; Response surface methodology; Wasted cooking oil.

MeSH terms

Adaptation, Physiological*
Hydrogen-Ion Concentration*
Opportunistic Infections / microbiology
Phylogeny
Pseudomonas Infections / microbiology
Pseudomonas aeruginosa / classification
Pseudomonas aeruginosa / physiology*
RNA, Ribosomal, 16S / genetics
Salinity*
Stress, Physiological*
Surface Tension
Surface-Active Agents / chemistry*
Surface-Active Agents / metabolism*
Temperature*

Substances

RNA, Ribosomal, 16S
Surface-Active Agents