Urinary oxylipin signature as biomarkers to monitor the allograft function during the first six months post-renal transplantation

Sonia Medina; Ignacio De Las Heras-Gómez; Teresa Casas-Pina; Valérie Bultel-Poncé; Jean-Marie Galano; Thierry Durand; Pedro Martínez-Hernández; Federico Ferreres; Luisa Jimeno; Santiago Llorente; Ángel Gil-Izquierdo

doi:10.1016/j.freeradbiomed.2019.11.010

Urinary oxylipin signature as biomarkers to monitor the allograft function during the first six months post-renal transplantation

Free Radic Biol Med. 2020 Jan:146:340-349. doi: 10.1016/j.freeradbiomed.2019.11.010. Epub 2019 Nov 15.

Affiliations

¹ Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100, Campus University Espinardo, Murcia, Spain. Electronic address: smescudero@cebas.csic.es.
² Clinical Analysis Service, University Hospital Virgen de la Arrixaca, Murcia, Ctra. Madrid-Cartagena, S/n, 30120, El Palmar, Spain.
³ Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 - CNRS, University of Montpellier - ENSCM, Faculty of Pharmacy, Montpellier, France.
⁴ Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100, Campus University Espinardo, Murcia, Spain.
⁵ Nephrology Service, University Hospital Virgen de la Arrixaca, Murcia, Ctra. Madrid-Cartagena, S/n, 30120, El Palmar, Spain.
⁶ Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100, Campus University Espinardo, Murcia, Spain. Electronic address: angelgil@cebas.csic.es.

PMID: 31734358
DOI: 10.1016/j.freeradbiomed.2019.11.010

Abstract

Oxylipins such as isoprostanes (IsoPs), prostaglandins (PGs) and thromboxanes (TXs) are lipid mediators derived from the oxidation of polyunsaturated fatty acids, which regulate the magnitude of oxidative stress and inflammation processes and play an important role in pathophysiological processes in the kidney. A total of 36 oxylipins were analyzed by UHPLC-QqQ-MS/MS in the urine of 41 renal recipients from cadaveric donors of the Nephrology Unit of the University Hospital Virgen de la Arrixaca during the first six months after renal transplantation, in order to investigate several candidate oxylipins as more accurate and predictive biomarkers in renal transplantation than classical biological variables. A decrease in nine PGs, mostly from the AA-D pathway (p < 0.05) and one IsoP: 15-keto-15-F_2t-IsoP (p < 0.001) was observed. Moreover, two PGs (2,3-dinor-11β-PGF_2α and 17-trans-PGF_3α) increased between five days and six months after renal transplantation (p < 0.05). In addition, when kidney function improved, a positive correlation between oxylipin levels and the excretion of urine proteins was observed. These results suggest that oxylipins could be useful markers for monitoring renal function in the post-renal transplantation period. These findings could be of utility not only for the development of strategies for long-term preservation of graft function, but also for innovative and alternative therapies -using oxylipins as predictive markers-to avoid organ rejection.

Keywords: Biomarkers; Inflammation; Isoprostanes; Lipidomics; Oxidative stress; Prostanoids; Renal transplant; Urine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allografts
Biomarkers
Humans
Kidney Transplantation* / adverse effects
Oxylipins*
Tandem Mass Spectrometry

Substances

Biomarkers
Oxylipins