Identification of miRNAs from stem cell derived microparticles in umbilical cord blood

Exp Hematol. 2019 Dec:80:21-26. doi: 10.1016/j.exphem.2019.11.001. Epub 2019 Nov 14.

Abstract

Umbilical cord blood CD34+ (UCB-CD34+) stem cells are clinically used in hematopoietic cell transplantation. However, there are limitations in the use of umbilical cord blood transplants because of the small number of cells and delayed engraftment. To gain a better understanding of functional components of UCB, we have detected and characterized CD34+ microparticles (CD34+MPs) from cord blood units. We collected cord blood units and assessed the numbers of CD34+MPs before and after red blood cell and plasma depletion by SEPAX processing using flow cytometry analysis. In parallel we identified MPs by electron microscopy. CD34+MPs and cells were isolated by MACs sorting. MicroRNAs (miR-106, miR-221, miR-517, miR-519, and miR-221) exhibited a characteristic microRNA profile that was further validated in isolated CD34+MPs. We found that in cord blood, there are CD34+MPs that carry microRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annexin A5 / analysis
  • Antigens, CD34 / analysis
  • Cell-Derived Microparticles* / chemistry
  • Cord Blood Stem Cell Transplantation / methods*
  • Fetal Blood / chemistry*
  • Hematopoietic Stem Cells / chemistry*
  • Hematopoietic Stem Cells / ultrastructure
  • Humans
  • Infant, Newborn
  • MicroRNAs / blood*
  • MicroRNAs / isolation & purification
  • Microscopy, Electron
  • Real-Time Polymerase Chain Reaction

Substances

  • Annexin A5
  • Antigens, CD34
  • MicroRNAs