Bioavailability and cytotoxicity of Cerium- (IV), Copper- (II), and Zinc oxide nanoparticles to human intestinal and liver cells through food

Abstract

Anthropogenic nanoparticles (NPs) are emitted to the environment and may be present in vegetables for human consumption. However, the toxicity of NPs exposure through food lack systematical investigations. In order to propose a systematical study, lettuce grown in a Cerium- (IV), Copper- (II) and Zinc oxide NP contaminated environment were digested. This digestate was used to culture human intestine cells (i.e. epithelial colorectal adenocarcinoma cells, Caco-2). The basolateral juice produced by the intestinal cells was then used to culture normal human liver (HL-7702) cells. Bioavailability and biotoxicity of the NPs in the vitro models were assessed. NPs were found to be taken up from the environment by vegetables, and may thus be transferred to humans through oral exposure. Bioavailability and the effect of their concentration in the digestate medium differed in regards to NP materials. The levels of NPs found in the digestate were detrimental to intestine cells, while the liver cells exposed to lower concentrations of NP in the bodily fluid showed no statically significant change in cell necrosis. A closer assessment of the detrimental effect of the studied NPs to Caco-2 cells revealed that the damage was mainly related to the solubility of the NPs. This may partly be due to that the more soluble NP material (ZnO > CuO > CeO₂) render higher metal ion release and thus higher bioavailability. This appeared to cause more cell death, and even lead to local intestinal inflammation. Although no liver cells died, there was an increase of ROS level, causing ROS-related DNA damage prior to cell necrosis. The findings in this study enhances our understanding of the relative detrimental effect of different types of NPs, and the mechanisms causing their biotoxicity in human cells through food.

Keywords: Bioavailability; Biotoxicity; In vitro; NPs in vegetable.