Molecular cloning and functional characterization of duck Janus kinase 1

Dejian Liu; Huijun Zheng; Yaqian Li; Peng Zhou; Hui Jin; Rui Luo

doi:10.1016/j.molimm.2019.10.022

Molecular cloning and functional characterization of duck Janus kinase 1

Mol Immunol. 2020 Jan:117:29-36. doi: 10.1016/j.molimm.2019.10.022. Epub 2019 Nov 13.

Authors

Dejian Liu¹, Huijun Zheng¹, Yaqian Li¹, Peng Zhou¹, Hui Jin¹, Rui Luo²

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China.
² State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, the Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei 430070, China. Electronic address: luorui@mail.hzau.edu.cn.

PMID: 31733446
DOI: 10.1016/j.molimm.2019.10.022

Abstract

Janus kinase 1 (JAK1) is a member of JAK family of non-receptor protein tyrosine kinases that plays critical roles in transducing cytokine signals via JAK-signal transducer and activator of transcription (STAT) signaling pathway. The importance of JAK1 in innate immunity has been well-studied in mammals and fish, yet in avian remains largely unknown. Here, we cloned the full-length of the duck JAK1 (duJAK1) gene for the first time. DuJAK1 encoded a protein of 1152 amino acids and possessed high amino acid identity with goose and budgerigar JAK1s. The duJAK1 was expressed in all detected tissues, especially high in the thymus and bursa of Fabricius. Overexpression of duJAK1 significantly activated ISRE promoter activity and induced duck viperin, 2', 5'-OAS, MX, PKR and ZAP expression. Knockdown of duJAK1 by small interfering RNA significantly inhibited duck Tembusu virus (DTMUV)-, duck Enteritis virus (DEV)-, poly (I:C)-, poly (dA:dT)- or Sendai virus (SeV)-induced ISRE promoter activation. Furthermore, duJAK1 exhibited antiviral activity against DTMUV infection. These results will help us understand the function of JAK family proteins in duck antiviral immunity.

Keywords: Duck; Innate immunity; JAK1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Avian Proteins / genetics
Avian Proteins / immunology*
Ducks / genetics
Ducks / immunology*
Immunity, Innate / genetics
Immunity, Innate / immunology*
Janus Kinase 1 / genetics
Janus Kinase 1 / immunology*

Substances

Avian Proteins
Janus Kinase 1