Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features

Long Huang; Zhiqiang Chen; Qinxue Wen; Ye Ji; Zening Wu; Duu-Jong Lee

doi:10.1016/j.biortech.2019.122369

Toward flexible regulation of polyhydroxyalkanoate composition based on substrate feeding strategy: Insights into microbial community and metabolic features

Bioresour Technol. 2020 Jan:296:122369. doi: 10.1016/j.biortech.2019.122369. Epub 2019 Nov 5.

Authors

Long Huang¹, Zhiqiang Chen², Qinxue Wen³, Ye Ji², Zening Wu¹, Duu-Jong Lee⁴

Affiliations

¹ School of Water Conservancy and Environment Engineering, Zhengzhou University, Zhengzhou 450002, China.
² State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
³ State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. Electronic address: wqxshelly@hit.edu.cn.
⁴ Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.

PMID: 31732415
DOI: 10.1016/j.biortech.2019.122369

Abstract

The suitable feeding strategy considering both substrate preference (enrichment stage) and flexible regulation (PHA accumulation stage) were investigated, respectively, based on intracellular polymers synthesis peculiarities of the three types of cultures (M-Ac, M-Pr and M-Bu), which were enriched correspondingly using acetic type, propionic type and butyric type substrate. Compared to M-Ac and M-Bu cultures, maximum PHA content (PHA_m) of M-Pr exhibited the most stable responses to varying fractions of propionic acid (f_Pr) of the substrate. The substrate composed of acetic acid and propionic acid (Mix-AP) demonstrated higher efficiency in regulation of polymer composition than that composed of butyric acid and propionic acid (Mix-BP). For the whole process of three-stage MC PHA production, propionic acid-dominated acidification products should be used for the long-term enrichment of PHA producers, and acidification products mainly composed of propionic and acetic acid are preferred considering the regulation of polymer composition in PHA accumulation stage.

Keywords: Community structure; Metabolic flux; Mixed culture (MC); Polyhydroxyalkanoate (PHA); Polymer composition.

MeSH terms

Acetic Acid
Bioreactors
Microbiota*
Polyhydroxyalkanoates*
Social Control, Formal

Substances

Polyhydroxyalkanoates
Acetic Acid