A mediator microbial biosensor for assaying general toxicity

A S Kharkova; V A Arlyapov; A D Turovskaya; V I Shvets; A N Reshetilov

doi:10.1016/j.enzmictec.2019.109435

A mediator microbial biosensor for assaying general toxicity

Enzyme Microb Technol. 2020 Jan:132:109435. doi: 10.1016/j.enzmictec.2019.109435. Epub 2019 Sep 19.

Authors

A S Kharkova¹, V A Arlyapov¹, A D Turovskaya², V I Shvets³, A N Reshetilov⁴

Affiliations

¹ FSBEIHE Tula State University, 92 Lenin Pr., Tula, 300012, Russia.
² FSBEIHE Tula State University, 92 Lenin Pr., Tula, 300012, Russia; FBI State Regional Centre of Standardization, Metrology and Testing in the Tula Region, 91 Boldin Str., Tula, 300028, Russia.
³ FSBEIHE Moscow Technological University, 86 Vernadsky Pr., Moscow, 119571, Russia.
⁴ FSBEIHE Tula State University, 92 Lenin Pr., Tula, 300012, Russia; FSBIS G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 5 Pr. Nauki, Pushchino, Moscow Region, 142290, Russia. Electronic address: anatol@ibpm.pushchino.ru.

PMID: 31731957
DOI: 10.1016/j.enzmictec.2019.109435

Abstract

A mediator biosensor based on Paracoccus yeei bacteria for assaying the toxicity of perfumery and cosmetics samples was developed. An approach to selecting an electron-transport mediator based on the heterogeneous electron transfer constants for investigated mediators (k_s) and the mediator-biomaterial interaction constants (k_interact) was proposed. Screening of nine compounds as potential mediators showed a ferrocene mediator immobilized in graphite paste to have the highest efficiency of electron transfer to the graphite-paste electrode (the heterogeneous transfer constant, 0.4 ± 0.1 cm/s) and a high constant of interaction with P. yeei (0.023 ± 0.001 dm³/(g·s)). A biosensor for toxicity assessment based on the ferrocene mediator and P. yeei bacteria was formed. The biosensor was tested on samples of four heavy metals (Cu²⁺, Zn²⁺, Pb²⁺, Cd²⁺) and two phenols (phenol and p-nitrophenol). Proceeding from the EC₅₀ index, it was found that the use of the ferrocene mediator made the biosensor more sensitive to investigated toxicants than most analogues described. Toxicity determination of four perfumery and cosmetics samples by the developed biosensor showed prospects of using this system for real-time toxicity monitoring of samples.

Keywords: Biosensor; Mediator; Paracoccus yeei bacteria; Toxicity; VKM B-3302; ЕC(50).

MeSH terms

Biosensing Techniques*
Metals, Heavy / analysis
Nitrophenols / analysis
Paracoccus / drug effects*
Perfume / analysis*
Phenols / analysis
Toxicity Tests / methods*

Substances

Metals, Heavy
Nitrophenols
Perfume
Phenols
4-nitrophenol

Supplementary concepts

Paracoccus yeei