The polysaccharide fraction of Bletilla ochracea (BOP) was isolated from its tubers, and purified by DEAE-52 Cellulose and Sephadex G-200 column chromatography. The structural features of BOP were analyzed by GC-MS, HPLP, FT-IR, methylation and NMR. The average molecular weight of BOP was approximately 4.9 × 105 Da, and the monosaccharide composition was 7.88:2.12 mix of mannose and glucose. The FT-IR and NMR analyses revealed the following linkage: [→(4-β-D-Man-1)4 → (4-β-D-Glc-1)2 → 4-2-O-acetyl-β-D-Man-1 → 4-β-D-Man-1 → 4-3-O-acetyl-β-D-Man-1 → 4-2-O-acetyl-β-D-Man-1→]. Although BOP had no effect on the proliferation rate of CT26 colon carcinoma cells, it significantly inhibited tumor xenograft growth in vivo by stimulating CD4+ T cell expansion in the spleen of the tumor-bearing mice. Taken together, BOP is a potent immunomodulatory agent that can be considered for anti-tumor therapy.
Keywords: Anti-tumor; Bletilla ochracea; Immuno-modulation; Polysaccharide; Structure.
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