Facile fabrication of a high-efficient and biocompatibility biocatalyst for bisphenol A removal

Lei Zhang; Jiali Mi; Guannan Hu; Chen Zhang; Haishan Qi

doi:10.1016/j.ijbiomac.2019.11.007

Facile fabrication of a high-efficient and biocompatibility biocatalyst for bisphenol A removal

Int J Biol Macromol. 2020 May 1:150:948-954. doi: 10.1016/j.ijbiomac.2019.11.007. Epub 2019 Nov 12.

Authors

Lei Zhang¹, Jiali Mi¹, Guannan Hu¹, Chen Zhang¹, Haishan Qi²

Affiliations

¹ Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of ChemicalScience and Engineering (Tianjin), Tianjin University, Tianjin 300072, China.
² Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of ChemicalScience and Engineering (Tianjin), Tianjin University, Tianjin 300072, China. Electronic address: hsqi@tju.edu.cn.

PMID: 31730948
DOI: 10.1016/j.ijbiomac.2019.11.007

Abstract

In this study, we developed a smart microfluidic device to prepare biocatalyst HRP-pCBMA. HRP-pCBMA is composed of horseradish peroxidase (HRP) and zwitterionic polymers poly(carboxybetaine methacrylate) (pCBMA), and could be applied to biodegrade bisphenol A (BPA) efficiently. Compared to free HRP, HRP-pCBMA exhibited an obviously enhanced degrading capability for 1 mM BPA with 99.42% degradation efficiency within 20 min, even being superior to 20-fold amount of free HPR. Besides, HRP-pCBMA displayed high stability against the abrupt changes of environmental factors (temperature, pH and organic solvents), and HRP-pCBMA exhibited a relatively high BPA degradation rate of more than 90% even after 10 cycles. The K_cat and V_max values of HRP-pCBMA were both 7-fold higher than that of free HRP, indicating significant improvement of the catalytic activity. Furthermore, the cytotoxicity assay indicated HRP-pCBMA has excellent biocompatibility. These results demonstrated that HRP-pCBMA possessed great potential in the bioremediation of BPA.

Keywords: Biocompatibility; Bisphenol A; Horseradish peroxidase; Microfluidic device; Poly(carboxybetaine methacrylate).

MeSH terms

Adsorption
Benzhydryl Compounds / chemistry
Benzhydryl Compounds / metabolism*
Biodegradation, Environmental*
Enzymes / chemistry*
Enzymes, Immobilized / metabolism
Horseradish Peroxidase / metabolism
Hydrogen-Ion Concentration
Lab-On-A-Chip Devices
Materials Testing
Methacrylates
Phenols / chemistry
Phenols / metabolism*
Polymers
Temperature

Substances

Benzhydryl Compounds
Enzymes
Enzymes, Immobilized
Methacrylates
Phenols
Polymers
Horseradish Peroxidase
bisphenol A