Three new prenyloxy chromanones from aerial parts of Hypericum aucheri

Abstract

Three new prenyloxy chromanone derivatives, aucherine A-C (6, 7 and 9) as well as six known prenylated phloroglucinols (1-5 and 8) were isolated from the aerial parts of Hypericum aucheri Jaub. Et Spach. The structures of the isolated compounds were established by means of spectral techniques (HRESIMS, 1D and 2D NMR). The new compounds were tested on а panel of human tumor cell line using MTT assay. All tested compounds exerted moderate cytotoxicity with IC₅₀ values ranging from 19.6 to 57.8 μM. The influence of the new compounds on some key signaling molecules (procaspase-9 and Bcl-xL), implicated in the regulation of programmed cell death was assessed by Western blot analysis.

Keywords: Aucherines; Bcl-xL; Chromanones; Cytotoxicity; Hypericum aucheri; Procaspase-9.