Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum

Xiaolong Hu; Kangli Wang; Miao Yu; Peixin He; Hanzhen Qiao; Huiru Zhang; Zichao Wang

doi:10.3390/biom9110730

Characterization and Antioxidant Activity of a Low-Molecular-Weight Xanthan Gum

Biomolecules. 2019 Nov 12;9(11):730. doi: 10.3390/biom9110730.

Authors

Xiaolong Hu¹, Kangli Wang¹, Miao Yu¹, Peixin He¹, Hanzhen Qiao², Huiru Zhang², Zichao Wang²

Affiliations

¹ College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China.
² College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.

Abstract

In the present work, a low-molecular-weight xanthan gum (LW-XG) was successfully obtained via biodegradation of commercial xanthan by the endophytic fungus Chaetomiumglobosum CGMCC 6882. The monosaccharide composition of LW-XG was glucose, mannose, and glucuronic acid in a molar ratio of 1.63:1.5:1.0. The molecular weight of LW-XG was 4.07 × 10⁴ Da and much smaller than that of commercial xanthan (2.95 × 10⁶ Da). Antioxidant assays showed that LW-XG had a good scavenging ability on DPPH radicals, superoxide anions, and hydroxyl radicals and good ferric reducing power. Moreover, LW-XG exhibited excellent protective effect on H₂O₂-injured Caco-2 cells. Results of this work suggested that LW-XG could be used in foods or pharmaceuticals to alleviate and resist the oxidative damage induced by the overproduction of reactive oxygen species.

Keywords: Caco-2 cells; FT-IR and NMR analysis; X-ray diffraction (XRD) analysis; Xanthan gum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants* / chemistry
Antioxidants* / pharmacology
Caco-2 Cells
Humans
Hydrogen Peroxide / pharmacology
Oxidation-Reduction / drug effects
Polysaccharides, Bacterial* / chemistry
Polysaccharides, Bacterial* / pharmacology

Substances

Antioxidants
Polysaccharides, Bacterial
Hydrogen Peroxide
xanthan gum