Metabolic profiling of Daphnia magna exposure to a mixture of hydrophobic organic contaminants in the presence of dissolved organic matter

Abstract

The hydrophobic organic contaminants triclosan, triphenyl phosphate (TPhP) and diazinon sorb to dissolved organic matter (DOM) and this may alter their bioavailability and toxicity. ¹H nuclear magnetic resonance (NMR)-based metabolomics was used to investigate how DOM at 1 and 5 mg organic carbon/L may alter the metabolome of Daphnia magna from exposure to equitoxic mixtures of triclosan, TPhP and diazinon. These contaminants have different modes of action toward D. magna. The contaminant concentrations in each mixture were an equal percentage of their lethal concentration to 50% of the population (LC₅₀) values, which equates to 1250 μg/L TPhP, 330 μg/L triclosan and 0.9 μg/L diazinon. The ternary mixture exposure at 1% LC₅₀ values did not alter the D. magna metabolome. Contaminant mixture exposures at 5%, 10%, and 15% LC₅₀ values decreased glucose, serine and glycine concentrations and increased asparagine and threonine concentrations, suggesting disruptions in energy metabolism. The contaminant mixture had a unique mode of action in D. magna and DOM at 1 and 5 mg organic carbon/L did not change this mode of action. The estimated sorption of triclosan, TPhP or diazinon to DOM at 1 or 5 mg organic carbon/L in this experimental design was calculated to be <50% for each contaminant. This suggests that the mode of action of the contaminant mixture was not altered by DOM because the two environmentally relevant concentrations of DOM may have not substantially altered contaminant bioavailability. Our results indicate that DOM may not inevitably mitigate or alter the sub-lethal toxicity of a mixture of hydrophobic organic contaminants. This indicates the complexity of predicting the molecular-level toxicity of environmental mixtures. For adequate risk assessment of freshwater ecosystems, it is vital to account for the combined sub-lethal toxicity of an environmental mixture of contaminants.

Keywords: Diazinon; NMR-based metabolomics; Triclosan; Triphenyl phosphate; Water flea.