Chrysanthemum (Chrysanthemum morifolium) CmICE2 conferred freezing tolerance in Arabidopsis

Zhaohe Zhang; Lu Zhu; Aiping Song; Haibin Wang; Sumei Chen; Jiafu Jiang; Fadi Chen

doi:10.1016/j.plaphy.2019.10.041

Chrysanthemum (Chrysanthemum morifolium) CmICE2 conferred freezing tolerance in Arabidopsis

Plant Physiol Biochem. 2020 Jan:146:31-41. doi: 10.1016/j.plaphy.2019.10.041. Epub 2019 Nov 2.

Authors

Zhaohe Zhang¹, Lu Zhu², Aiping Song³, Haibin Wang⁴, Sumei Chen⁵, Jiafu Jiang⁶, Fadi Chen⁷

Affiliations

¹ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: 2014204029@njau.edu.cn.
² State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: luzhu@jaas.ac.cn.
³ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: aiping_song@njau.edu.cn.
⁴ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: hb@njau.edu.cn.
⁵ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: chensm@njau.edu.cn.
⁶ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: jiangjiafu@njau.edu.cn.
⁷ State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: chenfd@njau.edu.cn.

PMID: 31726380
DOI: 10.1016/j.plaphy.2019.10.041

Abstract

Genes of the ICE (Inducer of CBF Expression) family play a key role in cold and freezing stresses response via the CBF regulatory pathway. In this work, we identified the ICE family gene, CmICE2, from Chrysanthemum morifolium 'Jinba'. CmICE2 encodes a 451-amino acid protein with a conserved nuclear localization domain, a bHLH domain and ACT domain. CmICE2 is expressed in abundance in leaves and flowers, and the expression of CmICE2 is induced by freezing and drought stresses. CmICE2 localized to the nucleus, and has transcriptional activity in yeast cells. After a 24-hour 4 °C acclimation, Arabidopsis plants overexpressing CmICE2 were more tolerant to freezing stress (-9 °C for 6 h) than the Col-0. When exposed to -9 °C for 6 h, the expression levels of genes such as AtCBF1, AtCBF2, AtCBF4, AtCOR 6.6A, AtCOR 414 and AtKIN1 were up-regulated significantly in CmICE2 overexpression plant lines compared to wild type. The proline contents, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were also increased in plants overexpressing CmICE2. In summary, CmICE2 confers to plant response to freezing stress.

Keywords: Chrysanthemum morifolium; Cold acclimation; Freezing stress; ICE; Transgenic Arabidopsis.

MeSH terms

Arabidopsis*
Chrysanthemum*
Flowers
Freezing
Gene Expression Regulation, Plant
Plant Proteins
Plants, Genetically Modified

Substances

Plant Proteins