Larval exposure to bacteria modulates arbovirus infection and immune gene expression in adult Aedes aegypti

Abstract

Here we have investigated whether bacterial challenges to larval stages of Aedes aegypti can influence the adults' immune and vector competence for dengue and Zika viruses. We show that larval exposure to live Bacillus thuringiensis Berliner and Enterobacter ludwigii can result in the modulation of virus infection at the adult stage in the absence of bacterial carry-over between the two developmental stages. We observed a significant reduction in virus infection intensity in the mosquitoes exposed to bacteria as larvae but not re-exposed as adults. The pattern of immune gene transcript regulation after bacterial exposure varied between adults, depending on whether or not they had been exposed to bacteria as larvae. Adults exposed to bacteria as larvae showed an earlier immune gene mRNA enrichment when re-exposed as adults than did adults not exposed as larvae. Bacterial exposure of larvae appears to have only modest effects on adult fitness.

Keywords: Aedes aegypti; Bacteria; Dengue virus; Innate immunity; Transstadial; Zika virus.