Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize

Nathan C Swyers; Jon P Cody; Morgan E McCaw; Nathaniel D Graham; Changzeng Zhao; Robert T Gaeta; James A Birchler

doi:10.1002/cppb.20031

Telomere-Mediated Chromosomal Truncation for Generating Engineered Minichromosomes in Maize

Curr Protoc Plant Biol. 2016 Sep;1(3):488-500. doi: 10.1002/cppb.20031.

Authors

Nathan C Swyers¹, Jon P Cody¹, Morgan E McCaw¹, Nathaniel D Graham¹, Changzeng Zhao¹, Robert T Gaeta¹, James A Birchler¹

Affiliation

¹ Division of Biological Sciences, University of Missouri, Columbia, Missouri.

PMID: 31725959
DOI: 10.1002/cppb.20031

Abstract

Minichromosomes have been generated in maize using telomere-mediated truncation. Telomere DNA, because of its repetitive nature, can be difficult to manipulate. The protocols in this unit describe two methods for generating the telomere DNA required for the initiation of telomere-mediated truncation. The resulting DNA can then be used with truncation cassettes for introduction into maize via transformation. © 2016 by John Wiley & Sons, Inc.

Keywords: minichromosomes; telomere cloning; telomere-mediated truncation.