Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells

Byeong Mun Kwak; Ee-Hwa Kim; Yong-Min Kim; Hee-Tack Kim

doi:10.12965/jer.1938442.221

Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells

J Exerc Rehabil. 2019 Oct 28;15(5):723-730. doi: 10.12965/jer.1938442.221. eCollection 2019 Oct.

Authors

Byeong Mun Kwak¹, Ee-Hwa Kim¹, Yong-Min Kim², Hee-Tack Kim³

Affiliations

¹ Department of Meridian & Acupoint, College of Korean Medicine, Semyung University, Jecheon, Korea.
² Department of Oriental Medical and Herbal Cosmetic Sciences, Semyung University, Jecheon, Korea.
³ Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University, Jecheon, Korea.

Abstract

The main purpose of this study was to suggest a solution for inflammation caused by urban daily life based on supercritical extraction of Chamaecyparis obtusa Endl. (C. obtusa). The leaves, cone, wood, and bark of C. obtusa were separately extracted with supercritical extraction. Alpha-pinene, anti-inflammatory active compound of C. obtusa, was analyzed by electrospray ionization-mass spectrometry, gas chromatography, and gas chromatography mass spectrometry from each part of extracts. As a result of the reduction rate of nitric oxide in RAW 264.7 cells, the wood extract exhibited the highest reduction rate was about 45% among extracts from four part of C. obtusa. The wood extract from C. obtusa can be developed as a good anti-inflammatory natural raw material for natural product industry.

Keywords: Anti-inflammatory effect; Chamaecyparis obtusa Endl.; Supercritical fluid extraction.