In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

José A Guijarro; Jessica Méndez

doi:10.1007/978-1-4939-9940-8_6

In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

Methods Mol Biol. 2020:2081:69-80. doi: 10.1007/978-1-4939-9940-8_6.

Authors

José A Guijarro¹, Jessica Méndez²

Affiliations

¹ Departamento de Biología Funcional, Facultad de Medicina, IUBA, Universidad de Oviedo, Oviedo, Asturias, Spain.
² Departamento de Biología Funcional, Facultad de Medicina, IUBA, Universidad de Oviedo, Oviedo, Asturias, Spain. yekamendez@yahoo.es.

PMID: 31721119
DOI: 10.1007/978-1-4939-9940-8_6

Abstract

Bioluminescent reporters and advanced luciferase technologies are useful to study host-pathogen interactions. This chapter describes the use of the luxCDABE operon from Photorhabdus luminescens as a tool to analyze the progression of the fish pathogen Yersinia ruckeri during the infection of rainbow trout, as well as the quantification of promoter activity of specific bacterial genes during host colonization.

Keywords: Bioluminescence imaging; IVIS® Imaging System; Infection process; Promoter activity; Rainbow trout; Yersinia ruckeri; luxCDABE operon.

MeSH terms

Animals
Fish Diseases / diagnosis*
Fish Diseases / microbiology*
Gene Expression
Gene Order
Genes, Reporter
Luminescent Measurements / methods*
Oncorhynchus mykiss / microbiology
Operon
Plasmids / genetics
Promoter Regions, Genetic
Yersinia Infections / veterinary*
Yersinia ruckeri*