Production of a New Cyclic Depsipeptide by the Culture Broth of Staphylococcus sp. Isolated from Corallina officinalis L

Reda F A Abdelhameed; Sameh S Elhady; Ahmad O Noor; Diena M Almasri; Alaa A Bagalagel; Galal T Maatooq; Amgad I M Khedr; Koji Yamada

doi:10.3390/metabo9110273

Production of a New Cyclic Depsipeptide by the Culture Broth of Staphylococcus sp. Isolated from Corallina officinalis L

Metabolites. 2019 Nov 11;9(11):273. doi: 10.3390/metabo9110273.

Authors

Reda F A Abdelhameed¹, Sameh S Elhady^{2

3}, Ahmad O Noor⁴, Diena M Almasri⁴, Alaa A Bagalagel⁴, Galal T Maatooq^{5

6}, Amgad I M Khedr³, Koji Yamada⁷

Affiliations

¹ Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
² Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
³ Department of Pharmacognosy, Faculty of Pharmacy, Port Said University, Port Said 42526, Egypt.
⁴ Department of Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
⁵ Department of Pharmacognosy, Faculty of Pharmacy, The Islamic University in Najaf, Najaf 54001, Iraq.
⁶ Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
⁷ Garden for Medicinal Plants, Graduate School of Biomedical Sciences, Nagasaki University; Bunkyo-machi 1-14, Nagasaki 852-8521, Japan.

Abstract

A new cyclic depsipeptide (1) has been isolated from culture broth of Staphylococcus sp. (No. P-100826-4-6) derived from Corallina officinalis L., together with the known compounds indol-3-carboxylic acid (2), 1,5-dideoxy-3-C-methyl arabinitol (3), thymine (4), uracil (5), cyclo (L-pro-L-omet) (6) and macrolactin B (7). The structure of (1) was established to be cyclo (2α, 3-diaminopropoinc acid-L-Asn-3-β-hydroxy-5-methyl-tetradecanoic acid-L-Leu¹-L-Asp-L-Val-L-Leu²-L-Leu³) by extensive spectroscopic techniques including ¹H NMR, ¹³C NMR, ¹H‒¹H COSY, HMBC, HSQC, NOESY, and HRFABMS. The antimicrobial activities of compounds 1-7 were evaluated. Compounds 1-5, and 7 showed moderate antimicrobial activity while compound 6 exhibited a potent antimicrobial and antifungal activities.

Keywords: Corallina officinalis; Staphylococcus sp.; antimicrobial assay; cyclic depsipeptide.