Promoting cellulase and hemicellulase production from Trichoderma orientalis EU7-22 by overexpression of transcription factors Xyr1 and Ace3

Yong Xue; Jiang Han; Yuyu Li; Jian Liu; Lihui Gan; Minnan Long

doi:10.1016/j.biortech.2019.122355

Promoting cellulase and hemicellulase production from Trichoderma orientalis EU7-22 by overexpression of transcription factors Xyr1 and Ace3

Bioresour Technol. 2020 Jan:296:122355. doi: 10.1016/j.biortech.2019.122355. Epub 2019 Nov 1.

Authors

Yong Xue¹, Jiang Han¹, Yuyu Li¹, Jian Liu², Lihui Gan³, Minnan Long⁴

Affiliations

¹ College of Energy, Xiamen University, Xiamen 361005, PR China.
² College of Energy, Xiamen University, Xiamen 361005, PR China; Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural Biomass, Xiamen University, Xiamen, PR China. Electronic address: jianliu@xmu.edu.cn.
³ College of Energy, Xiamen University, Xiamen 361005, PR China; Xiamen Key Laboratory of High-valued Conversion Technology of Agricultural Biomass, Xiamen University, Xiamen, PR China. Electronic address: ganlihui@xmu.edu.cn.
⁴ College of Energy, Xiamen University, Xiamen 361005, PR China; Fujian Vocational College of Bioengineering, Fuzhou, PR China. Electronic address: longmn@xmu.edu.cn.

PMID: 31711906
DOI: 10.1016/j.biortech.2019.122355

Abstract

The construction of hyper-production strains of cellulase is the prerequisite for the production of biofuels or biochemicals. Trichoderma orientalis EU7-22 with complete cellulase system shows the potential for cellulase production in industrial scale. To improve the cellulase production, two crucial transcription activators Xyr1 and Ace3 were constitutively overexpressed in EU7-22 strain. Cellulase, xylanase and protein secretion were significantly improved in the recombinant strain dxyA-8 under inducing culture, which were 2.34, 0.68 and 1.06 folds higher than those of EU7-22, respectively. Moreover, the FPase and CMCase activities were up to 2.55 IU/mL and 90.38 IU/mL with glucose as carbon source, which were 2.12 and 1.95 folds higher than those of EU7-22 under inducing condition, respectively. Reducing sugar released from pretreated spartina that hydrolyzed by crude enzyme from dxyA-8 had achieved 24% improvement. Therefore, overexpression of these transcription factors effectively promotes the production of cellulase and hemicellulase of Trichoderma orientalis EU7-22.

Keywords: Ace3; Cellulase; Overexpression; Trichoderma orientalis; Xyr1.

MeSH terms

Cellulase*
Glycoside Hydrolases
Transcription Factors
Trichoderma*

Substances

Transcription Factors
Glycoside Hydrolases
hemicellulase
Cellulase