Generation of 5 hiPSC lines derived from three unrelated idiopathic Parkinson disease patients and two unrelated healthy control individuals

Fabiano de Araújo Tofoli; Hsin Fen Chien; Egberto Reis Barbosa; Lygia V Pereira

doi:10.1016/j.scr.2019.101640

Generation of 5 hiPSC lines derived from three unrelated idiopathic Parkinson disease patients and two unrelated healthy control individuals

Stem Cell Res. 2019 Dec:41:101640. doi: 10.1016/j.scr.2019.101640. Epub 2019 Nov 4.

Authors

Fabiano de Araújo Tofoli¹, Hsin Fen Chien², Egberto Reis Barbosa², Lygia V Pereira³

Affiliations

¹ National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil.
² Movement Disorders Clinic Division of Neurology, Hospital das Clínicas, Faculty of Medicine, Universidade de São Paulo (HC-FMUSP), Brazil.
³ National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, SP 05508-900, Brazil. Electronic address: lpereira@usp.br.

PMID: 31710912
DOI: 10.1016/j.scr.2019.101640

Abstract

We describe generation of human induced pluripotent stem cell (hiPSC) lines of three unrelated idiopathic late onset Parkinson disease patients and two healthy controls above 60 years of age without neurological diseases nor Ashkenazi ancestry. Human iPSC were derived from peripheral blood-erythroblasts using integration free episomal plasmids carrying four reprogramming factors OCT4, SOX2, c-MYC, KLF4 and BCL-XL. The hiPSC lines were characterized according to established criteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Base Sequence
Cell Culture Techniques / methods*
Cell Line / pathology*
Humans
Induced Pluripotent Stem Cells / pathology*
Kruppel-Like Factor 4
Middle Aged
Parkinson Disease / pathology*
Reproducibility of Results