Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model

Methods Mol Biol. 2020:2086:237-250. doi: 10.1007/978-1-0716-0146-4_18.

Abstract

Immunotherapy has been showed as a promisor treatment, in special for hematological diseases. Chimeric antigen receptor T cells (CARs) which are showing satisfactory results in early-phase cancer clinical trials can be highlighted. However, preclinical models are critical steps prior to clinical trial. In this way, a well-established preclinical model is an important key in order to confirm the proof of principle. For this purpose, in this chapter will be pointed the methods to generate tumor cells expressing firefly Luciferase. In turn, these modified cells will be used to create a subcutaneous and a systemic murine model of Burkitt's lymphoma in order to evaluate the effectiveness of CAR-T.

Keywords: Bioluminescence imaging; CAR-T; Luciferase; Preclinical model; Subcutaneous model; Systemic model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Burkitt Lymphoma / diagnostic imaging
  • Burkitt Lymphoma / etiology
  • Burkitt Lymphoma / pathology
  • Burkitt Lymphoma / therapy
  • Cell Line, Tumor
  • Disease Models, Animal
  • Gene Expression*
  • Genes, Reporter*
  • Humans
  • Immunotherapy, Adoptive* / methods
  • Luciferases, Firefly / genetics
  • Mice
  • Molecular Imaging / methods
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / metabolism*
  • Receptors, Chimeric Antigen / genetics
  • Receptors, Chimeric Antigen / metabolism*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism*
  • Transduction, Genetic
  • Treatment Outcome
  • Xenograft Model Antitumor Assays

Substances

  • Receptors, Antigen, T-Cell
  • Receptors, Chimeric Antigen
  • Luciferases, Firefly