An efficient method for the development of myogenic differentiation using the stem cells can be beneficial in patients with severely compromised mobility, muscular damage, or degenerative diseases. The stem cells can prove to be excellent clinical source of myogenic progenitor cells due to their ability of self-proliferation, renewal, and differentiation into a specific phenotype. They represent an essential component of tissue engineering along with other factors (e.g., 3D scaffolds, ECM mimicking environment, and growth factors). In this chapter, we describe the experimental protocols for isolation of the embryonic stem cells, their proliferation on nanofiber scaffolds, and finally their differentiation into myogenic cells. Furthermore, this chapter elaborates experimental methods to assess the myogenic fate of embryonic stem cells on the nanofiber scaffolds.
Keywords: Extracellular substrate; Myogenic differentiation; Nanofibers; Wnt3a protein.