A novel LEAP-2 in diploid hybrid fish (Carassius auratus cuvieri ♀ × Carassius auratus red var. ♂) confers protection against bacteria-stimulated inflammatory response

Comp Biochem Physiol C Toxicol Pharmacol. 2020 Feb:228:108665. doi: 10.1016/j.cbpc.2019.108665. Epub 2019 Nov 7.

Abstract

LEAP-2, a multifunctional peptide, not only exhibits a regulatory role in pathogenic infection, but also participates in the regulation of teleostean immunity. In this study, ORF sequence of WR-LEAP-2 was 240 bp and encoded 79 amino acid residues. Tissue-specific analysis revealed that the highest expression of WR-LEAP-2 was observed in liver. Aeromonas hydrophila challenge can sharply increase WR-LEAP-2 mRNA expression in liver, kidney and spleen. The purified WR-LEAP-2 peptide can directly bind to A. hydrophila and S. agalactiae, reduce the relative bacterial activity and limit bacterial growth in vitro. In addition, the treatment of WR-LEAP-2 can restrict bacterial dissemination in vivo and reduce production of pro-inflammatory cytokines. These results indicated that WR-LEAP-2 can confer protection against A. hydrophila- or S. agalactiae-stimulated MyD88-dependent pro-inflammatory cytokines activation.

Keywords: Aeromonas hydrophila; Diploid hybrid fish; Gene expression; Inflammatory response; LEAP-2.

MeSH terms

  • Aeromonas hydrophila*
  • Animals
  • Antimicrobial Cationic Peptides / genetics
  • Antimicrobial Cationic Peptides / immunology*
  • Antimicrobial Cationic Peptides / pharmacology
  • Chimera / genetics
  • Chimera / immunology*
  • Cytokines / metabolism
  • Diploidy
  • Fish Diseases / genetics
  • Fish Diseases / microbiology*
  • Fish Proteins / metabolism*
  • Goldfish / genetics
  • Goldfish / immunology*
  • Gram-Negative Bacterial Infections / genetics
  • Gram-Negative Bacterial Infections / immunology*
  • Gram-Negative Bacterial Infections / veterinary*
  • Liver / immunology

Substances

  • Antimicrobial Cationic Peptides
  • Cytokines
  • Fish Proteins