Isolation, characterization and antioxidant of polysaccharides from Stropharia rugosoannulata

Ying Liu; Chao-Fan Hu; Xi Feng; Lei Cheng; Salam A Ibrahim; Cheng-Tao Wang; Wen Huang

doi:10.1016/j.ijbiomac.2019.11.045

Isolation, characterization and antioxidant of polysaccharides from Stropharia rugosoannulata

Int J Biol Macromol. 2020 Jul 15:155:883-889. doi: 10.1016/j.ijbiomac.2019.11.045. Epub 2019 Nov 7.

Authors

Ying Liu¹, Chao-Fan Hu², Xi Feng³, Lei Cheng⁴, Salam A Ibrahim⁵, Cheng-Tao Wang⁶, Wen Huang⁷

Affiliations

¹ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
² College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
³ Department of Nutrition, Food Science and Packaging, California State University, San Jose, CA 95192, United States.
⁴ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China.
⁵ Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, United States.
⁶ Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China. Electronic address: wctbtbu@hotmail.com.
⁷ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: huangwen@mail.hzau.edu.cn.

PMID: 31706814
DOI: 10.1016/j.ijbiomac.2019.11.045

Abstract

In this study, polysaccharides from Stropharia rugosoannulata were isolated and purified by macroporous adsorption resin and ion exchange chromatography to produce SRP-1 and SRP-2. Preliminary structural characteristics of SRP-1 and SRP-2 were also investigated. Our results showed that SRP-1 was neutral, while SRP-2 was acidic polysaccharides. The molar ratios of monosaccharides in SRP-1 and SRP-2 were different, but glucose was the main monosaccharide. Both α-type and β-type glycosidic linkages were detected in SRP-1, whereas β-constitution was dominant in SRP-2. Both SRP-1 and SRP-2 had a (1 → 6)-α-D-glucan backbone. Furthermore, the antioxidant activities of polysaccharides against ABTS⁺, DPPH and OH^- radicals were determined in vitro. The results indicated that SRP-2 had a stronger antioxidant capacity than that of SRP-1. Our results may be valuable with regard to the utilization of polysaccharides from S. rugosoannulata as potential pharmaceuticals.

Keywords: Antioxidant capacity; Characterization; Isolation; Polysaccharides; Stropharia rugosoannulata.

MeSH terms

Agaricales / classification*
Antioxidants* / chemistry
Antioxidants* / isolation & purification
Carbohydrate Sequence
Fungal Polysaccharides* / chemistry
Fungal Polysaccharides* / isolation & purification

Substances

Antioxidants
Fungal Polysaccharides

Supplementary concepts

Stropharia rugosoannulata