Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor

Gabriele Louise Soares Martins; Bruno Diaz Paredes; Gabriela Louise de Almeida Sampaio; Carolina Kymie Vasques Nonaka; Katia Nunes da Silva; Kyan James Allahdadi; Luciana Souza de Aragão França; Milena Botelho Pereira Soares; Ricardo Ribeiro Dos Santos; Bruno Solano de Freitas Souza

doi:10.1016/j.scr.2019.101630

Generation of human iPS cell line CBTCi001-A from dermal fibroblasts obtained from a healthy donor

Stem Cell Res. 2019 Dec:41:101630. doi: 10.1016/j.scr.2019.101630. Epub 2019 Oct 21.

Affiliations

¹ Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil.
² Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.
³ Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.
⁴ Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, RJ, Brazil.
⁵ Center for Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, Brazil; Gonçalo Moniz Institute, FIOCRUZ, Salvador, BA, Brazil; D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil; National Institute of Science and Technology for Regenerative Medicine, Rio de Janeiro, RJ, Brazil. Electronic address: bruno.solano@fiocruz.br.

PMID: 31706097
DOI: 10.1016/j.scr.2019.101630

Abstract

Human-induced pluripotent stem cell (hiPSC) CBTCi001-A line was generated from a healthy 30-year old male dermal fibroblasts using non-integrative reprogramming method using episomal-based plasmids expressing OCT4, SOX2, KLF4, and MYCL. Characterization of CBTCi001-A was confirmed by the expression of typical markers of pluripotency and differentiation potential in vitro.

MeSH terms

Adult
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line / cytology*
Dermis / cytology*
Fibroblasts / cytology*
Humans
Induced Pluripotent Stem Cells / cytology*
Kruppel-Like Factor 4
Male
Reproducibility of Results
Tissue Donors*