Co-valorization of paper mill sludge and corn steep liquor for enhanced n-butanol production with Clostridium tyrobutyricum Δcat1::adhE2

Xianshuang Cao; Zhu Chen; Liyan Liang; Liang Guo; Zhihua Jiang; Feng Tang; Yang Yun; Yi Wang

doi:10.1016/j.biortech.2019.122347

Co-valorization of paper mill sludge and corn steep liquor for enhanced n-butanol production with Clostridium tyrobutyricum Δcat1::adhE2

Bioresour Technol. 2020 Jan:296:122347. doi: 10.1016/j.biortech.2019.122347. Epub 2019 Oct 28.

Authors

Xianshuang Cao¹, Zhu Chen², Liyan Liang³, Liang Guo⁴, Zhihua Jiang⁵, Feng Tang⁶, Yang Yun⁷, Yi Wang⁸

Affiliations

¹ Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA; SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, Beijing 100714, China.
² Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA.
³ Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA; College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi 030006, China.
⁴ College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
⁵ Department of Chemical Engineering, Auburn University, Auburn, AL 36849, USA.
⁶ SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, Beijing 100714, China.
⁷ College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi 030006, China.
⁸ Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA; Center for Bioenergy and Bioproducts, Auburn University, Auburn, AL 36849, USA. Electronic address: yiwang3@auburn.edu.

PMID: 31704602
DOI: 10.1016/j.biortech.2019.122347

Abstract

In this study, hyper-butanol producing Clostridium tyrobutyricum Δcat1::adhE2 was used for butanol production from paper mill sludge (PMS) and corn steep liquor (CSL). Our results demonstrated that CSL can not only serve as a cheap nitrogen source, but also provide lactic acid that can be assimilated by C. tyrobutyricum for enhanced butanol production. Through a separate hydrolysis and fermentation, 16.5 g/L butanol with a yield of 0.26 g/g was obtained from PMS hydrolysates supplemented with 5% CSL. Further, a separate repeated hydrolysis was conducted to improve PMS hydrolysis rate and enhance sugar yield. Fermentation using hydrolysates from such process also generated high-level butanol with high yield. Our results suggested an innovative bioprocess for efficient biobutanol production from low-value waste streams.

Keywords: Butanol; Clostridium tyrobutyricum; Corn steep liquor; Lactic acid; Paper mill sludge.

MeSH terms

1-Butanol
Butanols
Clostridium
Clostridium tyrobutyricum*
Fermentation
Sewage
Zea mays

Substances

Butanols
Sewage
1-Butanol