Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis

Ivana Kolić; Ljiljana Stojković; Evica Dinčić; Ivan Jovanović; Aleksandra Stanković; Maja Živković

doi:10.1016/j.jneuroim.2019.577090

Expression of LEP, LEPR and PGC1A genes is altered in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis

J Neuroimmunol. 2020 Jan 15:338:577090. doi: 10.1016/j.jneuroim.2019.577090. Epub 2019 Oct 26.

Authors

Ivana Kolić¹, Ljiljana Stojković², Evica Dinčić³, Ivan Jovanović⁴, Aleksandra Stanković⁵, Maja Živković⁶

Affiliations

¹ "Vinča" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, Serbia. Electronic address: ivanak@vin.bg.ac.rs.
² "Vinča" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, Serbia. Electronic address: ljiljanas@vin.bg.ac.rs.
³ Military Medical Academy, Clinic for Neurology, Crnotravska 17, Belgrade, Serbia.
⁴ "Vinča" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, Serbia. Electronic address: ivanj@vin.bg.ac.rs.
⁵ "Vinča" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, Serbia. Electronic address: alexas@vin.bg.ac.rs.
⁶ "Vinča" Institute of Nuclear Sciences, Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Mike Petrovića Alasa 12-14, Belgrade, Serbia. Electronic address: majaz@vin.bg.ac.rs.

PMID: 31704454
DOI: 10.1016/j.jneuroim.2019.577090

Abstract

Leptin (LEP) may contribute to the pathogenesis of multiple sclerosis (MS) by its immunomodulatory, proinflammatory and prooxidant effects. Therefore, plasma LEP levels and mRNA expression of five genes related to the LEP signaling pathway (LEP, LEP receptor (LEPR), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1A), superoxide dismutase 2, tumor necrosis factor-alpha) were investigated in relapsing-remitting MS. In patients (N = 64), compared to healthy subjects (N = 62), relative LEP mRNA levels were significantly increased (p = 0,01), while LEPR and PGC1A mRNA levels were decreased (p = 0,001 and p = 0,04, respectively). Significant positive correlation was observed between LEPR mRNA levels and clinical parameters of MS progression (EDSS, MSSS).

Keywords: Inflammation; Leptin; Multiple sclerosis; Oxidative response; Peripheral blood mononuclear cells; mRNA expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Female
Humans
Leptin / blood
Leptin / genetics*
Leukocytes, Mononuclear / metabolism*
Male
Middle Aged
Multiple Sclerosis, Relapsing-Remitting / etiology
Multiple Sclerosis, Relapsing-Remitting / metabolism*
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / blood
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / genetics*
RNA, Messenger / analysis
Reactive Oxygen Species / metabolism
Receptors, Leptin / blood
Receptors, Leptin / genetics*
Tumor Necrosis Factor-alpha / genetics
Young Adult

Substances

Leptin
PPARGC1A protein, human
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
RNA, Messenger
Reactive Oxygen Species
Receptors, Leptin
Tumor Necrosis Factor-alpha