Cytological examination of cerebrospinal fluid: Sysmex UF-1000i versus optical microscopy

Anal Biochem. 2020 Jan 15:589:113501. doi: 10.1016/j.ab.2019.113501. Epub 2019 Nov 5.

Abstract

We evaluated the body fluid module on Sysmex UF-1000i (UF-1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid. We collected 93 cerebrospinal fluid samples and compared the results of the UF-1000i-BF mode with the Fast-Read 102 disposable counting cell. Results shows a good correlation between the UF-1000i and the microscopic examination. The concordance percentage is 99.06% for white blood cells and 85.18% for red blood cells. The UF-1000i-BF mode offers rapid and reliable total WBC and RBC counts for initial screening of cerebrospinal fluid, and can improve the workflow in a routine laboratory.

Keywords: Body fluid; Cytology; Red blood cell; White blood cell.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Body Fluids / cytology*
  • Child
  • Child, Preschool
  • Erythrocyte Count* / instrumentation
  • Erythrocyte Count* / methods
  • Erythrocytes / cytology*
  • Humans
  • Infant
  • Leukocyte Count* / instrumentation
  • Leukocyte Count* / methods
  • Leukocytes / cytology*
  • Microscopy / methods
  • Middle Aged
  • Prospective Studies
  • Young Adult