Investigation of lipoic acid - 4-methoxybenzyl alcohol reaction and evaluation of its analytical usefulness

Monika Turkowicz; Izabella Jastrzebska; Marta Hryniewicka; Urszula Kotowska; Diana Gudalewska; Joanna Karpińska

doi:10.1016/j.foodchem.2019.125750

Investigation of lipoic acid - 4-methoxybenzyl alcohol reaction and evaluation of its analytical usefulness

Food Chem. 2020 Mar 30:309:125750. doi: 10.1016/j.foodchem.2019.125750. Epub 2019 Oct 23.

Authors

Monika Turkowicz¹, Izabella Jastrzebska², Marta Hryniewicka², Urszula Kotowska², Diana Gudalewska², Joanna Karpińska²

Affiliations

¹ Institute of Chemistry, University of Białystok, ul. Ciołkowskiego 1K, 15-245 Białystok, Poland. Electronic address: monika.turkowicz@wp.pl.
² Institute of Chemistry, University of Białystok, ul. Ciołkowskiego 1K, 15-245 Białystok, Poland.

PMID: 31704079
DOI: 10.1016/j.foodchem.2019.125750

Abstract

The presented work is aimed to synthesize a new UV active derivative of α-lipoic acid (ALA) by its esterification with 4-methoxybenzyl alcohol (4-MBA, anise alcohol). The formation of ester was confirmed by ¹HNMR, FTIR and UV spectroscopy. The analytical usefulness of the obtained compound for quantification of ALA in food items was examined using HPLC-UV and GC-MS systems. It was found that it is possible to assay ALA in the ester form in the concentration ranges: 5·10^-6-1·10^-4 mol L^-1 by HPLC-UV and 1∙10^-7-5∙10^-5 mol L^-1 by GC-MS techniques. The GC-MS procedure was applied for the determination of ALA in the food samples.

Keywords: 4-methoxybenzyl alcohol; Chromatographic analysis; Derivatization reaction; Lipoic acid.

MeSH terms

Anisoles / chemistry*
Chromatography, High Pressure Liquid / methods
Esterification
Gas Chromatography-Mass Spectrometry / methods
Thioctic Acid / chemistry*

Substances

Anisoles
Thioctic Acid
anisyl alcohol