The purpose of this paper is to establish a method for easy operation, high sensitivity, strong anti-interference ability, and rapid quantitative detection of cardiac fatty acid-binding protein in acute myocardial infarction biomarkers, so that it can be quickly diagnosed at an early stage and provide a basis for further treatment. Based on the SERS principle, the traditional sandwich system generated by the reaction was captured by the streptavidin (SA) magnetic beads through the specific reaction of SA and biotin then enriched by the applied magnetic field. The enriched magnetic beads are subjected to Raman detection to achieve a process of quantitative detection of the antigen. The minimum detection limit of this study was 1.4490 ng ml-1, the recoveries were 97.36%-98.35%, and the relative standard deviations between batches and batches were less than 15%. There was no crossover between cTnI, D-dimmer and NT-proBNP. In addition to hemoglobin, the common interfering substances in serum and common anticoagulants do not interfere with the test results. Surface-enhanced Raman spectroscopy can quickly and accurately quantify the acute myocardial infarction marker H-FABP, which is easy to operate and strong in anti-interference ability.