Abstract
One hallmark of protein chemical synthesis is its capacity to access proteins that living systems can hardly produce. This is typically the case for proteins harboring post-translational modifications such as ubiquitin or ubiquitin-like modifiers. Various methods have been developed for accessing polyubiquitin conjugates by semi- or total synthesis. Comparatively, the preparation of small-ubiquitin-like modifier (SUMO) conjugates, and more particularly of polySUMO scaffolds, is much less developed. We describe hereinafter a synthetic strategy for accessing all SUMO-2/3 dimer combinations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Humans
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Peptide Fragments / chemistry*
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Polymers / chemistry*
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Polyubiquitin / metabolism*
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Protein Conformation
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Protein Multimerization*
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Protein Processing, Post-Translational
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Sequence Homology
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Small Ubiquitin-Related Modifier Proteins / chemical synthesis*
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Small Ubiquitin-Related Modifier Proteins / metabolism
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Ubiquitins / chemical synthesis*
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Ubiquitins / metabolism
Substances
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Peptide Fragments
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Polymers
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SUMO2 protein, human
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SUMO3 protein, human
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Small Ubiquitin-Related Modifier Proteins
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Ubiquitins
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Polyubiquitin