Luteimonas lumbrici sp. nov., a novel bacterium isolated from wormcast

Abstract

A Gram-stain-negative, yellow-green bacterium, designated 1.1416^T, was isolated from wormcast of Eisenia foetida. The strain was non-motile, rod-shaped, and grew optimally on NA medium at 30 °C, pH 7.0 and with 0 % (w/v) NaCl. On the basis of the 16S rRNA gene sequence and phylogenetic analysis, 1.1416^T showed the highest degree of 16S rRNA gene sequence similarity to Luteimonas arsenica 26-35^T (96.2 %), followed by Luteimonas lutimaris G3^T (96.1 %). The respiratory quinone of 1.1416^T was ubiquinone-8 (Q-8), and its major cellular fatty acids were iso-C_{15 : 0} (39.8 %), summed feature 9 (iso-C_{17 : 1} ω9c or C_{16 : 0} 10-methyl) (18.6 %). The major polar lipids of 1.1416^T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and six unidentified phospholipids. The genomic DNA G+C content of 1.1416^T was 71.0 mol%. According to the results of the phenotypic and chemotaxonomic phylogenetic analyses, strain 1.1416^T represents a novel species of the genus Luteimonas, for which the name Luteimonas lumbrici sp. nov. is proposed, with strain 1.1416^T (=KCTC 62979^T=CCTCC AB 2018348^T) as the type strain.

Keywords: 16S rRNA gene; Luteimonas lumbrici sp. nov.; polyphasic taxonomic analysis; wormcast.