Antagonistic and plant-growth promoting novel Bacillus species from long-term organic farming soils from Sikkim, India

Periyasamy Panneerselvam; Ansuman Senapati; Upendra Kumar; Laxuman Sharma; Pinky Lepcha; S R Prabhukarthikeyan; Afrin Jahan; Chidambaram Parameshwaran; Guru Prasana Pandi Govindharaj; Srikanta Lenka; Prafulla Kumar Nayak; Debasis Mitra; Mahapatra Smruthi Sagarika; Sugitha Thangappan; Utthandi Sivakumar

doi:10.1007/s13205-019-1938-7

Antagonistic and plant-growth promoting novel Bacillus species from long-term organic farming soils from Sikkim, India

3 Biotech. 2019 Nov;9(11):416. doi: 10.1007/s13205-019-1938-7. Epub 2019 Oct 23.

Authors

Periyasamy Panneerselvam¹, Ansuman Senapati¹, Upendra Kumar¹, Laxuman Sharma², Pinky Lepcha², S R Prabhukarthikeyan¹, Afrin Jahan¹, Chidambaram Parameshwaran¹, Guru Prasana Pandi Govindharaj¹, Srikanta Lenka¹, Prafulla Kumar Nayak¹, Debasis Mitra², Mahapatra Smruthi Sagarika¹, Sugitha Thangappan³, Utthandi Sivakumar³

Affiliations

¹ 1Crop Production Division, ICAR-National Rice Research Institute, Cuttack, 753006 Odisha India.
² 2Department of Horticulture, Sikkim University, Gangtok, 737102 Sikkim India.
³ 3Tamil Nadu Agricultural University, Coimbatore, 641003 Tamil Nadu India.

Abstract

Three bacteria namely Bacillus luciferensis K2, Bacillus amyloliquefaciens K12 and Bacillus subtilis BioCWB possessing plant growth promotion and biocontrol potential against phytopathogens and rice leaf folder were identified from organic soils of Sikkim, India. The results revealed significant higher production of phytohormones IAA (97.1 μg mL^-1) and GA₃ (10.6 μg mL^-1) was found in K2, whereas BioCWB had higher phosphate solubilization (570.0 μg mL^-1) efficacy and also possessed nitrogen fixation ability (5.34 log copy number mL^-1 culture). All these bacteria had higher antagonistic activities against phytopathogens viz. Rhizoctonia solani, Fusarium proliferatum, Athelia rolfsii and Colletotrichum gloeosporioides and also had higher larvicidal activity against rice leaf folder Cnaphalocrocis medinalis (Guenne) under in vitro conditions. Molecular insights into the antagonistic mechanisms of Bacillus strains deciphered the presence of several antimicrobial peptides (ericin, subtilin, surfactin, iturin, bacilysin, subtilosin, fengycin and bacillomycin), volatiles (dimethyl disulphide, methyl-Furan, acetic acid, Z-1,3-pentadiene and 3-hexyn-2-ol) and soluble metabolites (9-octadecenamide, E-15-heptadecenal, E-3-eicosene and 5-octadecene). Furthermore, liquid microbial inoculum prepared using the bacterial strains (K2, K12 and BioCWB) were evaluated under glass house (rice) and field condition (capsicum), which significantly enhanced plant growth in rice and yield in capsicum compared to control. The present study revealed the combination of Bacillus spp. (K2, K12 and BioCWB) can be used as bio-inoculants for improving agricultural production in Sikkim. Moreover, for the first time, we demonstrated plant growth promoting (PGP) traits, antifungal and insecticidal properties of B. luciferensis.

Keywords: Bacillus spp.; Biocontrol; PGP traits; Sikkim organic soil.