A Ratiometric and Colorimetric Hemicyanine Fluorescent Probe for Detection of SO2 Derivatives and Its Applications in Bioimaging

Molecules. 2019 Nov 5;24(21):4011. doi: 10.3390/molecules24214011.

Abstract

Based upon the intramolecular charge transfer (ICT) mechanism, a novel ratiometric fluorescent probe EB was developed to detect SO32-/HSO3-. The probe displayed both colorimetric and ratiometric responses toward SO32-/HSO3-. It displayed a quick response (within 60 s), good selectivity and high sensitivity (a detection limit of 28 nM) towards SO32-/HSO3-. The SO32-/HSO3- sensing mechanism was confirmed as the Michael addition reaction by ESI-MS. Moreover, the probe could be applied to measure the level of sulfite in real samples, like sugar and chrysanthemum, and it could also be used to detect SO32-/HSO3- in HepG2 cells through confocal fluorescence microscopy, which proved its practical application in clinical diagnosis.

Keywords: SO2 derivatives; bioimaging; ratiometric fluorescent probe.

MeSH terms

  • Carbocyanines / chemistry*
  • Cell Line, Tumor
  • Colorimetry / methods
  • Fluorescence
  • Fluorescent Dyes / chemistry*
  • Hep G2 Cells
  • Humans
  • Limit of Detection
  • Sensitivity and Specificity
  • Sulfites / chemistry
  • Sulfur Dioxide / chemistry*

Substances

  • Carbocyanines
  • Fluorescent Dyes
  • Sulfites
  • tetramethylene hemicyanine
  • Sulfur Dioxide