BET bromodomain inhibition suppresses adipogenesis in mice

Qiong Duan; Pei Wu; Zhenzhen Liu; Fan Xia; Lingyan Zhu; Zeqi Zheng; Tianlun Yang; Jun Qi

doi:10.1007/s12020-019-02115-4

BET bromodomain inhibition suppresses adipogenesis in mice

Endocrine. 2020 Jan;67(1):264-267. doi: 10.1007/s12020-019-02115-4. Epub 2019 Nov 5.

Authors

Qiong Duan^{1

2}, Pei Wu³, Zhenzhen Liu³, Fan Xia^{4

5}, Lingyan Zhu^{3

6}, Zeqi Zheng^{4

5}, Tianlun Yang⁷, Jun Qi^{8

9}

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Nanchang University, Yongwaizheng Street, Nanchang, China. qiongduan@csu.edu.cn.
² Jiangxi Hypertension Research Institute, Nanchang, China. qiongduan@csu.edu.cn.
³ Department of Cardiology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, China.
⁴ Department of Cardiology, The First Affiliated Hospital of Nanchang University, Yongwaizheng Street, Nanchang, China.
⁵ Jiangxi Hypertension Research Institute, Nanchang, China.
⁶ Department of Endocrinology, The First Affiliated Hospital of Nanchang University, Yongwaizheng Street, Nanchang, China.
⁷ Department of Endocrinology, The First Affiliated Hospital of Nanchang University, Yongwaizheng Street, Nanchang, China. tianluny@163.com.
⁸ Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA, USA. Jun_Qi@DFCI.HARVARD.EDU.
⁹ Department of Medicine, Harvard Medical School, Boston, MA, USA. Jun_Qi@DFCI.HARVARD.EDU.

PMID: 31691152
DOI: 10.1007/s12020-019-02115-4

Abstract

Purpose: We recently reported that inhibition of BET bromodomain suppresses adipogenesis in vitro. In the present study we aimed to address whether BET bromodomain inhibition can suppress adipogenesis in vivo.

Methods: Brd4^fl/fl mice were crossed with B6.Cg-Tg(Fabp4-cre)1Rev/J mice to generate Brd4^fl/+/Fabp4-cre mice. We used high fat diet (HFD, 45% fat) mice treated with vehicle (DMSO) or JQ1 (intraperitoneal, IP injection, 50 mg/kg/day), respectively, for 6 weeks. Body weight was measured once a week. Dual-energy X-ray absorptiometry was determined and brown adipose tissue was harvested at the end of the experiment.

Results: Partial deletion of Brd4 leads to the lower body weight. JQ1 treatment further confirmed that BET bromodomain inhibition suppresses body weight gain and decreases white adipose depots compared with the control mice. In addition, JQ1 treatment reduces the size of brown adipose tissue and impairs its thermogenesis.

Conclusions: BET bromodomain inhibition suppresses adipogenesis in the mice.

Keywords: Adipogenesis; BET bromodomain; Epigenetics; JQ1.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Adipogenesis*
Animals
Azepines / pharmacology
Mice
Nuclear Proteins* / genetics
Transcription Factors
Triazoles / pharmacology

Substances

Azepines
Nuclear Proteins
Transcription Factors
Triazoles

Abstract

Publication types

MeSH terms

Substances

Grants and funding